Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?
| Main Author: | |
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| Publication Date: | 2020 |
| Other Authors: | , , , , , , , , , , , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) |
| Download full: | http://hdl.handle.net/10451/45056 |
Summary: | © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?Extracellular vesiclesLung cancerProteomicsImmuno oncologyBronchoalveolar lavage© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).Acellular bronchoalveolar lavage (BAL) proteomics can partially separate lung cancer from non-lung cancer patients based on principal component analysis and multivariate analysis. Furthermore, the variance in the proteomics data sets is correlated mainly with lung cancer status and, to a lesser extent, smoking status and gender. Despite these advances BAL small and large extracellular vehicles (EVs) proteomes reveal aberrant protein expression in paracrine signaling mechanisms in cancer initiation and progression. We consequently present a case-control study of 24 bronchoalveolar lavage extracellular vesicle samples which were analyzed by state-of-the-art liquid chromatography-mass spectrometry (LC-MS). We obtained evidence that BAL EVs proteome complexity correlated with lung cancer stage 4 and mortality within two years´ follow-up (p value = 0.006). The potential therapeutic target DNMT3B complex is significantly up-regulated in tumor tissue and BAL EVs. The computational analysis of the immune and fibroblast cell markers in EVs suggests that patients who deceased within the follow-up period display higher marker expression indicative of innate immune and fibroblast cells (four out of five cases). This study provides insights into the proteome content of BAL EVs and their correlation to clinical outcomes.R.M. is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019–2025 investigator).This article is a result of the projects (iNOVA4Health—UID/Multi/04462/2013), supported by Lisboa PortugalRegional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through theEuropean Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE2020 Programme and National Funds through FCT—Portuguese Foundation for Science and Technology underthe projects number PTDC/BTM-TEC/30087/2017 and PTDC/BTM-TEC/30088/2017. This work was supported bythe Wellcome Trust/DBT India Alliance Margdarshi Fellowship (grant number IA/M/15/1/502023) awarded to A.P.B.C.-S., M.C.S.C. and C.B. are supported by the Champalimaud Foundation and the EMBO Installation Grant 3921MDPIRepositório da Universidade de LisboaCarvalho, Ana SofiaMoraes, Maria Carolina StranoHyun Na, ChanFierro-Monti, IvoHenriques, AndreiaZahedi, SaraBodo, CristianTranfield, Erin MSousa, Ana LauraFarinho, AnaRodrigues, Luís VazPinto, PaulaBárbara, CristinaMota, LeonorAbreu, Tiago Tavares deSemedo, JúlioSeixas, SusanaKumar, PrashantCosta-Silva, BrunoPandey, AkhileshMatthiesen, Rune2020-11-27T12:33:53Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/45056engCancers 2020, 12(11), 345010.3390/cancers121134502072-6694info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T14:24:59Zoai:repositorio.ulisboa.pt:10451/45056Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:11:19.402335Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse |
| dc.title.none.fl_str_mv |
Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer? |
| title |
Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer? |
| spellingShingle |
Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer? Carvalho, Ana Sofia Extracellular vesicles Lung cancer Proteomics Immuno oncology Bronchoalveolar lavage |
| title_short |
Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer? |
| title_full |
Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer? |
| title_fullStr |
Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer? |
| title_full_unstemmed |
Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer? |
| title_sort |
Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer? |
| author |
Carvalho, Ana Sofia |
| author_facet |
Carvalho, Ana Sofia Moraes, Maria Carolina Strano Hyun Na, Chan Fierro-Monti, Ivo Henriques, Andreia Zahedi, Sara Bodo, Cristian Tranfield, Erin M Sousa, Ana Laura Farinho, Ana Rodrigues, Luís Vaz Pinto, Paula Bárbara, Cristina Mota, Leonor Abreu, Tiago Tavares de Semedo, Júlio Seixas, Susana Kumar, Prashant Costa-Silva, Bruno Pandey, Akhilesh Matthiesen, Rune |
| author_role |
author |
| author2 |
Moraes, Maria Carolina Strano Hyun Na, Chan Fierro-Monti, Ivo Henriques, Andreia Zahedi, Sara Bodo, Cristian Tranfield, Erin M Sousa, Ana Laura Farinho, Ana Rodrigues, Luís Vaz Pinto, Paula Bárbara, Cristina Mota, Leonor Abreu, Tiago Tavares de Semedo, Júlio Seixas, Susana Kumar, Prashant Costa-Silva, Bruno Pandey, Akhilesh Matthiesen, Rune |
| author2_role |
author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
| dc.contributor.author.fl_str_mv |
Carvalho, Ana Sofia Moraes, Maria Carolina Strano Hyun Na, Chan Fierro-Monti, Ivo Henriques, Andreia Zahedi, Sara Bodo, Cristian Tranfield, Erin M Sousa, Ana Laura Farinho, Ana Rodrigues, Luís Vaz Pinto, Paula Bárbara, Cristina Mota, Leonor Abreu, Tiago Tavares de Semedo, Júlio Seixas, Susana Kumar, Prashant Costa-Silva, Bruno Pandey, Akhilesh Matthiesen, Rune |
| dc.subject.por.fl_str_mv |
Extracellular vesicles Lung cancer Proteomics Immuno oncology Bronchoalveolar lavage |
| topic |
Extracellular vesicles Lung cancer Proteomics Immuno oncology Bronchoalveolar lavage |
| description |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
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2020 |
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2020-11-27T12:33:53Z 2020 2020-01-01T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://hdl.handle.net/10451/45056 |
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http://hdl.handle.net/10451/45056 |
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eng |
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eng |
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Cancers 2020, 12(11), 3450 10.3390/cancers12113450 2072-6694 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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MDPI |
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MDPI |
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