Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?

Bibliographic Details
Main Author: Carvalho, Ana Sofia
Publication Date: 2020
Other Authors: Moraes, Maria Carolina Strano, Na, Chan Hyun, Fierro-Monti, Ivo, Henriques, Andreia, Zahedi, Sara, Bodo, Cristian, Tranfield, Erin M., Sousa, Ana Laura, Farinho, Ana, Rodrigues, Luís Vaz, Pinto, Paula, Bárbara, Cristina, Mota, Leonor, de Abreu, Tiago Tavares, Semedo, Júlio, Seixas, Susana, Kumar, Prashant, Costa-Silva, Bruno, Pandey, Akhilesh, Matthiesen, Rune
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/109483
Summary: Funding: R.M. is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019–2025 investigator). This article is a result of the projects (iNOVA4Health—UID/Multi/04462/2013), supported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT—Portuguese Foundation for Science and Technology under the projects number PTDC/BTM-TEC/30087/2017 and PTDC/BTM-TEC/30088/2017. This work was supported by the Wellcome Trust/DBT India Alliance Margdarshi Fellowship (grant number IA/M/15/1/502023) awarded to A.P. B.C.-S., M.C.S.C. and C.B. are supported by the Champalimaud Foundation and the EMBO Installation Grant 3921.
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spelling Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?Bronchoalveolar lavageExtracellular vesiclesImmuno oncologyLung cancerProteomicsOncologyCancer ResearchSDG 3 - Good Health and Well-beingFunding: R.M. is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019–2025 investigator). This article is a result of the projects (iNOVA4Health—UID/Multi/04462/2013), supported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT—Portuguese Foundation for Science and Technology under the projects number PTDC/BTM-TEC/30087/2017 and PTDC/BTM-TEC/30088/2017. This work was supported by the Wellcome Trust/DBT India Alliance Margdarshi Fellowship (grant number IA/M/15/1/502023) awarded to A.P. B.C.-S., M.C.S.C. and C.B. are supported by the Champalimaud Foundation and the EMBO Installation Grant 3921.Acellular bronchoalveolar lavage (BAL) proteomics can partially separate lung cancer from non-lung cancer patients based on principal component analysis and multivariate analysis. Furthermore, the variance in the proteomics data sets is correlated mainly with lung cancer status and, to a lesser extent, smoking status and gender. Despite these advances BAL small and large extracellular vehicles (EVs) proteomes reveal aberrant protein expression in paracrine signaling mechanisms in cancer initiation and progression. We consequently present a case-control study of 24 bronchoalveolar lavage extracellular vesicle samples which were analyzed by state-of-the-art liquid chromatography-mass spectrometry (LC-MS). We obtained evidence that BAL EVs proteome complexity correlated with lung cancer stage 4 and mortality within two years´ follow-up (p value = 0.006). The potential therapeutic target DNMT3B complex is significantly up-regulated in tumor tissue and BAL EVs. The computational analysis of the immune and fibroblast cell markers in EVs suggests that patients who deceased within the follow-up period display higher marker expression indicative of innate immune and fibroblast cells (four out of five cases). This study provides insights into the proteome content of BAL EVs and their correlation to clinical outcomes.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)iNOVA4Health - pólo NMSRUNCarvalho, Ana SofiaMoraes, Maria Carolina StranoNa, Chan HyunFierro-Monti, IvoHenriques, AndreiaZahedi, SaraBodo, CristianTranfield, Erin M.Sousa, Ana LauraFarinho, AnaRodrigues, Luís VazPinto, PaulaBárbara, CristinaMota, Leonorde Abreu, Tiago TavaresSemedo, JúlioSeixas, SusanaKumar, PrashantCosta-Silva, BrunoPandey, AkhileshMatthiesen, Rune2020-12-30T05:04:00Z2020-112020-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article18application/pdfhttp://hdl.handle.net/10362/109483eng2072-6694PURE: 26662759https://doi.org/10.3390/cancers12113450info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T17:49:36Zoai:run.unl.pt:10362/109483Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:20:56.453571Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?
title Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?
spellingShingle Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?
Carvalho, Ana Sofia
Bronchoalveolar lavage
Extracellular vesicles
Immuno oncology
Lung cancer
Proteomics
Oncology
Cancer Research
SDG 3 - Good Health and Well-being
title_short Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?
title_full Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?
title_fullStr Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?
title_full_unstemmed Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?
title_sort Is the proteome of bronchoalveolar lavage extracellular vesicles a marker of advanced lung cancer?
author Carvalho, Ana Sofia
author_facet Carvalho, Ana Sofia
Moraes, Maria Carolina Strano
Na, Chan Hyun
Fierro-Monti, Ivo
Henriques, Andreia
Zahedi, Sara
Bodo, Cristian
Tranfield, Erin M.
Sousa, Ana Laura
Farinho, Ana
Rodrigues, Luís Vaz
Pinto, Paula
Bárbara, Cristina
Mota, Leonor
de Abreu, Tiago Tavares
Semedo, Júlio
Seixas, Susana
Kumar, Prashant
Costa-Silva, Bruno
Pandey, Akhilesh
Matthiesen, Rune
author_role author
author2 Moraes, Maria Carolina Strano
Na, Chan Hyun
Fierro-Monti, Ivo
Henriques, Andreia
Zahedi, Sara
Bodo, Cristian
Tranfield, Erin M.
Sousa, Ana Laura
Farinho, Ana
Rodrigues, Luís Vaz
Pinto, Paula
Bárbara, Cristina
Mota, Leonor
de Abreu, Tiago Tavares
Semedo, Júlio
Seixas, Susana
Kumar, Prashant
Costa-Silva, Bruno
Pandey, Akhilesh
Matthiesen, Rune
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
Centro de Estudos de Doenças Crónicas (CEDOC)
iNOVA4Health - pólo NMS
RUN
dc.contributor.author.fl_str_mv Carvalho, Ana Sofia
Moraes, Maria Carolina Strano
Na, Chan Hyun
Fierro-Monti, Ivo
Henriques, Andreia
Zahedi, Sara
Bodo, Cristian
Tranfield, Erin M.
Sousa, Ana Laura
Farinho, Ana
Rodrigues, Luís Vaz
Pinto, Paula
Bárbara, Cristina
Mota, Leonor
de Abreu, Tiago Tavares
Semedo, Júlio
Seixas, Susana
Kumar, Prashant
Costa-Silva, Bruno
Pandey, Akhilesh
Matthiesen, Rune
dc.subject.por.fl_str_mv Bronchoalveolar lavage
Extracellular vesicles
Immuno oncology
Lung cancer
Proteomics
Oncology
Cancer Research
SDG 3 - Good Health and Well-being
topic Bronchoalveolar lavage
Extracellular vesicles
Immuno oncology
Lung cancer
Proteomics
Oncology
Cancer Research
SDG 3 - Good Health and Well-being
description Funding: R.M. is supported by Fundação para a Ciência e a Tecnologia (CEEC position, 2019–2025 investigator). This article is a result of the projects (iNOVA4Health—UID/Multi/04462/2013), supported by Lisboa Portugal Regional Operational Programme (Lisboa2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This work is also funded by FEDER funds through the COMPETE 2020 Programme and National Funds through FCT—Portuguese Foundation for Science and Technology under the projects number PTDC/BTM-TEC/30087/2017 and PTDC/BTM-TEC/30088/2017. This work was supported by the Wellcome Trust/DBT India Alliance Margdarshi Fellowship (grant number IA/M/15/1/502023) awarded to A.P. B.C.-S., M.C.S.C. and C.B. are supported by the Champalimaud Foundation and the EMBO Installation Grant 3921.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-30T05:04:00Z
2020-11
2020-11-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/109483
url http://hdl.handle.net/10362/109483
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2072-6694
PURE: 26662759
https://doi.org/10.3390/cancers12113450
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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