Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections

Bibliographic Details
Main Author: Melo, Luís Daniel Rodrigues
Publication Date: 2016
Other Authors: Veiga, Patrícia, Cerca, Nuno, Kropinski, Andrew M., Almeida, Carina, Azeredo, Joana, Sillankorva, Sanna
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/1822/42165
Summary: "Article 1024"
id RCAP_51dffe879125c4d99a78b711a6aaeba1
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/42165
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infectionsBacteriophagesBacteriophage therapyBiofilmsUrinary tract infectionProteus mirabilisPhage cocktailScience & Technology"Article 1024"Proteus mirabilis is an enterobacterium that causes catheter-associated urinary tract infections (CAUTIs) due to its ability to colonize and form crystalline biofilms on the catheters surface. CAUTIs are very difficult to treat, since biofilm structures are highly tolerant to antibiotics. Phages have been used widely to control a diversity of bacterial species, however a limited number of phages for P. mirabilis have been isolated and studied. Here we report the isolation of two novel virulent phages, the podovirus vB_PmiP_5460 and the myovirus vB_PmiM_5461, which are able to target respectively, 16 of the 26 and all the Proteus strains tested in this study. Both phages have been characterized thoroughly and sequencing data revealed no traces of genes associated with lysogeny. To further evaluate the phages ability to prevent catheter´s colonization by Proteus, the phages adherence to silicone surfaces was assessed. Further tests in phage-coated catheters using a dynamic biofilm model simulating CAUTIs, have shown a significant reduction of P. mirabilis biofilm formation up to 168 h of catheterization. These results highlight the potential usefulness of the two isolated phages for the prevention of surface colonization by this bacterium.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI- 01-0145-FEDER-006684) and by the Portuguese Foundation for Science and Technology (FCT) under the scope of the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER- 027462). NC and SS also thank FCT for the individual support through Investigador FCT contracts.Frontiers MediaUniversidade do MinhoMelo, Luís Daniel RodriguesVeiga, PatríciaCerca, NunoKropinski, Andrew M.Almeida, CarinaAzeredo, JoanaSillankorva, Sanna20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/42165engMelo, L.; Veiga, P.; Cerca N; Kropinski, Andrew M.; Almeida, Carina; Azeredo, Joana; Sillankorva, Sanna, Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections. Frontiers in Microbiology, 7(1024), 20161664-302X1664-302X10.3389/fmicb.2016.01024http://journal.frontiersin.org/journal/microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T06:37:41Zoai:repositorium.sdum.uminho.pt:1822/42165Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:59:20.588606Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections
title Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections
spellingShingle Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections
Melo, Luís Daniel Rodrigues
Bacteriophages
Bacteriophage therapy
Biofilms
Urinary tract infection
Proteus mirabilis
Phage cocktail
Science & Technology
title_short Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections
title_full Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections
title_fullStr Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections
title_full_unstemmed Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections
title_sort Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections
author Melo, Luís Daniel Rodrigues
author_facet Melo, Luís Daniel Rodrigues
Veiga, Patrícia
Cerca, Nuno
Kropinski, Andrew M.
Almeida, Carina
Azeredo, Joana
Sillankorva, Sanna
author_role author
author2 Veiga, Patrícia
Cerca, Nuno
Kropinski, Andrew M.
Almeida, Carina
Azeredo, Joana
Sillankorva, Sanna
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Melo, Luís Daniel Rodrigues
Veiga, Patrícia
Cerca, Nuno
Kropinski, Andrew M.
Almeida, Carina
Azeredo, Joana
Sillankorva, Sanna
dc.subject.por.fl_str_mv Bacteriophages
Bacteriophage therapy
Biofilms
Urinary tract infection
Proteus mirabilis
Phage cocktail
Science & Technology
topic Bacteriophages
Bacteriophage therapy
Biofilms
Urinary tract infection
Proteus mirabilis
Phage cocktail
Science & Technology
description "Article 1024"
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/42165
url http://hdl.handle.net/1822/42165
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Melo, L.; Veiga, P.; Cerca N; Kropinski, Andrew M.; Almeida, Carina; Azeredo, Joana; Sillankorva, Sanna, Development of a phage cocktail to control Proteus mirabilis catheter-associated urinary tract infections. Frontiers in Microbiology, 7(1024), 2016
1664-302X
1664-302X
10.3389/fmicb.2016.01024
http://journal.frontiersin.org/journal/microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833595664765812736

Similar Items