Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans

Bibliographic Details
Main Author: Marques, Cátia Filipa Saraiva
Publication Date: 2019
Other Authors: Belas, Adriana, Aboim, Catarina, Trigueiro, Graça, Cavaco-Silva, Patrícia, Gama, Luis, Pomba, C.
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.5/22146
Summary: Research Areas: Microbiology. Veterinary Sciences
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spelling Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humansProteus mirabilisUrinary tract infectionCompanion animalHumanClonal relatednessResearch Areas: Microbiology. Veterinary SciencesABSTRACT - Proteus mirabilis is a major cause of urinary tract infection (UTI) in humans and companion animals. This study aimed to evaluate the antimicrobial resistance, virulence and clonal relatedness of P. mirabilis isolated from dogs, cats and humans with UTI. P. mirabilis isolated from companion animals (N = 107) and humans (N = 76) with UTI were compared by PFGE analysis after overnight Nod macro-restriction using Dice/UPGMA with a 1.5% tolerance. Strains were characterized for antimicrobial resistance by disk diffusion. Twenty-four resistance genes and four virulence genes were screened by PCR. Thirty-nine clusters (similarity > 80%) and 73 single pulse-types were detected. Nine clusters included P. mirabilis isolated from community and hospital patients, including strains with 100% similarity. A high number of clusters (43.6%, n = 17/39) included strains from companion animals and humans. Similarity between some companion animal and human strains varied between 80-100%. One strain from a dog was 100% similar to one human community-acquired P. mirabilis. One P. mirabilis from a cat was found to be 94.7% and 92.4% similar to community and hospital patient strains, respectively. P. mirabilis CMY-2-producers did not cluster all together. Nevertheless, cluster C36 included five P. mirabilis from companion animals (similarity 85.8%-95.7%), of which, four (80%) were multidrug-resistant CMY-2-producers. This study shows that companion animals and humans become infected with closely related P. mirabilis strains. The high number of clusters containing companion animals and human strains points to the zoonotic nature of P. mirabilis. These results underline the potential role of companion animals as reservoirs and in the dissemination of uropathogenic P. mirabilis to humans and vice versa.ELSEVIERRepositório da Universidade de LisboaMarques, Cátia Filipa SaraivaBelas, AdrianaAboim, CatarinaTrigueiro, GraçaCavaco-Silva, PatríciaGama, LuisPomba, C.2021-10-06T15:08:42Z2019-012019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22146engMarques C, Belas A, Aboim C, Trigueiro G, Cavaco-Silva P, Gama LT, Pomba C. 2019. Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans. Veterinary Microbiology. 228(1):77-820378-113510.1016/j.vetmic.2018.10.015info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T15:23:50Zoai:repositorio.ulisboa.pt:10400.5/22146Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:42:17.554284Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
title Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
spellingShingle Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
Marques, Cátia Filipa Saraiva
Proteus mirabilis
Urinary tract infection
Companion animal
Human
Clonal relatedness
title_short Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
title_full Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
title_fullStr Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
title_full_unstemmed Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
title_sort Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
author Marques, Cátia Filipa Saraiva
author_facet Marques, Cátia Filipa Saraiva
Belas, Adriana
Aboim, Catarina
Trigueiro, Graça
Cavaco-Silva, Patrícia
Gama, Luis
Pomba, C.
author_role author
author2 Belas, Adriana
Aboim, Catarina
Trigueiro, Graça
Cavaco-Silva, Patrícia
Gama, Luis
Pomba, C.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Marques, Cátia Filipa Saraiva
Belas, Adriana
Aboim, Catarina
Trigueiro, Graça
Cavaco-Silva, Patrícia
Gama, Luis
Pomba, C.
dc.subject.por.fl_str_mv Proteus mirabilis
Urinary tract infection
Companion animal
Human
Clonal relatedness
topic Proteus mirabilis
Urinary tract infection
Companion animal
Human
Clonal relatedness
description Research Areas: Microbiology. Veterinary Sciences
publishDate 2019
dc.date.none.fl_str_mv 2019-01
2019-01-01T00:00:00Z
2021-10-06T15:08:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22146
url http://hdl.handle.net/10400.5/22146
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Marques C, Belas A, Aboim C, Trigueiro G, Cavaco-Silva P, Gama LT, Pomba C. 2019. Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans. Veterinary Microbiology. 228(1):77-82
0378-1135
10.1016/j.vetmic.2018.10.015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ELSEVIER
publisher.none.fl_str_mv ELSEVIER
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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