Behaviour Phenotyping of Parkinson’s Disease Animal Models Using Inertial Sensors

Bibliographic Details
Main Author: Saraiva, Tatiana Margarida Lança
Publication Date: 2021
Format: Master thesis
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/49401
Summary: Tese de mestrado Bioquímica (Bioquímica Médica) Universidade de Lisboa, Faculdade de Ciências, 2021
id RCAP_488a6ba1e1a2cecc2c24d40754d50973
oai_identifier_str oai:repositorio.ulisboa.pt:10451/49401
network_acronym_str RCAP
network_name_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository_id_str https://opendoar.ac.uk/repository/7160
spelling Behaviour Phenotyping of Parkinson’s Disease Animal Models Using Inertial SensorsDoença de ParkinsonDopaminaMitoParkSensores de InérciaLevodopaTeses de mestrado - 2021Departamento de Química e BioquímicaTese de mestrado Bioquímica (Bioquímica Médica) Universidade de Lisboa, Faculdade de Ciências, 2021Parkinson’s Disease is a progressive degenerative illness of the human nervous system that affects basal ganglia circuits. It is characterized by the loss of dopaminergic neurons in the substantia nigra pars compacta, loss of dopaminergic terminals in the striatum and widespread intracellular α-synuclein accumulation. Parkinson’s disease patients present three main motor symptoms: bradykinesia, muscular rigidity and tremor. Nevertheless, patients can also have additional motor and non-motor symptoms, such as apathy, depression, constipation, sleep behavior disorders, loss of sense of smell and cognitive impairment. At the time patients are first diagnosed, the loss of dopaminergic neurons in the substantia nigra pars compacta is already marked and the neurodegeneration has spread to other central nervous system regions. Nowadays, the most effective therapeutic strategy for Parkinson is the administration of Levodopa, a precursor of dopamine, that restores dopaminergic transmission in the nigrostriatal pathway. Nevertheless, Levodopa does not have an effect on the progression of the disease and its continuous administration induces the appearance of dyskinesias. These factors illustrate the importance of searching for novel ways of assessing these disease manifestations at early stages and why finding biomarkers that can reflect the disease progression would play an important role in clinical trials and in research using animal models, that are essential for the testing of new therapeutic approaches. As mentioned above, one way to study the degenerative process leading to Parkinson is using animal models. The MitoPark mice is an animal model for Parkinson Disease that mimics the progressive degeneration of the dopaminergic system through the knockout of the gene for mitochondrial transcription factor A (Tfam) in dopaminergic neurons using the Cre-loxP system. These mice lacking Tfam develop progressive respiratory chain deficiency followed by cell death. As a consequence of the progressive loss of dopaminergic neurons these mice develop Parkinson’s disease-like motor disabilities with ageing. In this project, we accessed both normal and Levodopa-induced behaviour of MitoPark mice using inertial sensors combined with video recordings. The use of inertial sensors allows the acquisition of high-resolution data, bringing the possibility of detecting behaviour patterns as the dopaminergic loss progresses that can be missed by the traditional simple video-tracking approach. Using these sensors we found a new biomarker to track the disease progression in this model that is based on the frequency domain of acceleration recordings. In addition to it, although the MitoPark mice responded to Levodopa with an increase in their acceleration and speed, the Levodopa treatment was not able to decrease the frequency domain of acceleration to control-like values. Altogether, our work shows that motion sensors are adequate for tracking disease progression and allow the measure of novel biomarkers, which may lead to a step further in the current endeavour of searching for novel therapeutic strategies for PD.Silva, Joaquim Alves daFerreira, António Eduardo do Nascimento, 1964-Repositório da Universidade de LisboaSaraiva, Tatiana Margarida Lança2024-06-18T00:30:43Z202120212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/49401TID:202934551enginfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T14:36:48Zoai:repositorio.ulisboa.pt:10451/49401Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:17:00.897462Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Behaviour Phenotyping of Parkinson’s Disease Animal Models Using Inertial Sensors
title Behaviour Phenotyping of Parkinson’s Disease Animal Models Using Inertial Sensors
spellingShingle Behaviour Phenotyping of Parkinson’s Disease Animal Models Using Inertial Sensors
Saraiva, Tatiana Margarida Lança
Doença de Parkinson
Dopamina
MitoPark
Sensores de Inércia
Levodopa
Teses de mestrado - 2021
Departamento de Química e Bioquímica
title_short Behaviour Phenotyping of Parkinson’s Disease Animal Models Using Inertial Sensors
title_full Behaviour Phenotyping of Parkinson’s Disease Animal Models Using Inertial Sensors
title_fullStr Behaviour Phenotyping of Parkinson’s Disease Animal Models Using Inertial Sensors
title_full_unstemmed Behaviour Phenotyping of Parkinson’s Disease Animal Models Using Inertial Sensors
title_sort Behaviour Phenotyping of Parkinson’s Disease Animal Models Using Inertial Sensors
author Saraiva, Tatiana Margarida Lança
author_facet Saraiva, Tatiana Margarida Lança
author_role author
dc.contributor.none.fl_str_mv Silva, Joaquim Alves da
Ferreira, António Eduardo do Nascimento, 1964-
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Saraiva, Tatiana Margarida Lança
dc.subject.por.fl_str_mv Doença de Parkinson
Dopamina
MitoPark
Sensores de Inércia
Levodopa
Teses de mestrado - 2021
Departamento de Química e Bioquímica
topic Doença de Parkinson
Dopamina
MitoPark
Sensores de Inércia
Levodopa
Teses de mestrado - 2021
Departamento de Química e Bioquímica
description Tese de mestrado Bioquímica (Bioquímica Médica) Universidade de Lisboa, Faculdade de Ciências, 2021
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021-01-01T00:00:00Z
2024-06-18T00:30:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/49401
TID:202934551
url http://hdl.handle.net/10451/49401
identifier_str_mv TID:202934551
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833601651208880128

Similar Items