Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells

Bibliographic Details
Main Author: Pereira, Inês Serrano
Publication Date: 2024
Other Authors: da Cunha, Maria, Leal, Inês Pacheco, Luís, Maria Pequito, Gonçalves, Paula, Gonçalves, Carla, Mota, Luís Jaime
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/173345
Summary: Funding Information: We are grateful to Mariana Mend\u00E3o for the construction of plasmids, and to our colleagues Agathe Subtil, Ian Clarke, Mar\u00EDa Rosa Vergara, and Nicole Borel for generously providing DNA of several Chlamydia species and antibodies. Funding Information: This work was supported by Funda\u00E7\u00E3o para a Ci\u00EAncia e Tecnologia (FCT) through grant PTDC/BIA-MIC/28503/2017, and in the scope of the projects UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences \u2013 UCIBIO, and LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. ISP and MPL were supported by PhD fellowships SFRH/BD/129756/2017 and SFRH/BD/144284/2019, respectively, also funded by FCT. Open access funding provided by FCT|FCCN (b-on) Publisher Copyright: © The Author(s) 2024.
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spelling Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cellsBacterial pathogenesisChlamydiaEffector evolutionPhylogenyType III secretionImmunology and AllergyImmunologyMicrobiology (medical)Funding Information: We are grateful to Mariana Mend\u00E3o for the construction of plasmids, and to our colleagues Agathe Subtil, Ian Clarke, Mar\u00EDa Rosa Vergara, and Nicole Borel for generously providing DNA of several Chlamydia species and antibodies. Funding Information: This work was supported by Funda\u00E7\u00E3o para a Ci\u00EAncia e Tecnologia (FCT) through grant PTDC/BIA-MIC/28503/2017, and in the scope of the projects UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences \u2013 UCIBIO, and LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. ISP and MPL were supported by PhD fellowships SFRH/BD/129756/2017 and SFRH/BD/144284/2019, respectively, also funded by FCT. Open access funding provided by FCT|FCCN (b-on) Publisher Copyright: © The Author(s) 2024.Chlamydiae are a large group of obligate endosymbionts of eukaryotes that includes the Chlamydiaceae family, comprising several animal pathogens. Among Chlamydiaceae, Chlamydia trachomatis causes widespread ocular and urogenital infections in humans. Like many bacterial pathogens, all Chlamydiae manipulate host cells by injecting them with type III secretion effector proteins. We previously characterized the C. trachomatis effector CteG, which localizes at the host cell Golgi and plasma membrane during distinct phases of the chlamydial infectious cycle. Here, we show that CteG is a Chlamydiaceae-specific effector with over 60 homologs phylogenetically categorized into two distinct clades (CteG I and CteG II) and exhibiting several inparalogs and outparalogs. Notably, cteG I homologs are syntenic to C. trachomatis cteG, whereas cteG II homologs are syntenic among themselves but not with C. trachomatis cteG. This indicates a complex evolution of cteG homologs, which is unique among C. trachomatis effectors, marked by numerous events of gene duplication and loss. Despite relatively modest sequence conservation, nearly all tested CteG I and CteG II proteins were identified as type III secretion substrates using Yersinia as a heterologous bacterial host. Moreover, most of the type III secreted CteG I and CteG II homologs were delivered by C. trachomatis into host cells, where they localized at the Golgi region and cell periphery. Overall, this provided insights into the evolution of bacterial effectors and revealed a Chlamydiaceae family of type III secreted proteins that underwent substantial divergence during evolution while conserving the capacity to localize at specific host cell compartments.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNPereira, Inês Serranoda Cunha, MariaLeal, Inês PachecoLuís, Maria PequitoGonçalves, PaulaGonçalves, CarlaMota, Luís Jaime2024-10-10T22:41:15Z2024-122024-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article19application/pdfhttp://hdl.handle.net/10362/173345engPereira, I. S., da Cunha, M., Leal, I. P., Luís, M. P., Gonçalves, P., Gonçalves, C., & Mota, L. J. (2024). Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells. Medical Microbiology and Immunology, 213(1), Article 15. Advance online publication. https://doi.org/10.1007/s00430-024-00798-90300-8584PURE: 100978246https://doi.org/10.1007/s00430-024-00798-9info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-01-20T01:36:40Zoai:run.unl.pt:10362/173345Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T18:59:05.791837Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells
title Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells
spellingShingle Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells
Pereira, Inês Serrano
Bacterial pathogenesis
Chlamydia
Effector evolution
Phylogeny
Type III secretion
Immunology and Allergy
Immunology
Microbiology (medical)
title_short Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells
title_full Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells
title_fullStr Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells
title_full_unstemmed Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells
title_sort Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells
author Pereira, Inês Serrano
author_facet Pereira, Inês Serrano
da Cunha, Maria
Leal, Inês Pacheco
Luís, Maria Pequito
Gonçalves, Paula
Gonçalves, Carla
Mota, Luís Jaime
author_role author
author2 da Cunha, Maria
Leal, Inês Pacheco
Luís, Maria Pequito
Gonçalves, Paula
Gonçalves, Carla
Mota, Luís Jaime
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Pereira, Inês Serrano
da Cunha, Maria
Leal, Inês Pacheco
Luís, Maria Pequito
Gonçalves, Paula
Gonçalves, Carla
Mota, Luís Jaime
dc.subject.por.fl_str_mv Bacterial pathogenesis
Chlamydia
Effector evolution
Phylogeny
Type III secretion
Immunology and Allergy
Immunology
Microbiology (medical)
topic Bacterial pathogenesis
Chlamydia
Effector evolution
Phylogeny
Type III secretion
Immunology and Allergy
Immunology
Microbiology (medical)
description Funding Information: We are grateful to Mariana Mend\u00E3o for the construction of plasmids, and to our colleagues Agathe Subtil, Ian Clarke, Mar\u00EDa Rosa Vergara, and Nicole Borel for generously providing DNA of several Chlamydia species and antibodies. Funding Information: This work was supported by Funda\u00E7\u00E3o para a Ci\u00EAncia e Tecnologia (FCT) through grant PTDC/BIA-MIC/28503/2017, and in the scope of the projects UIDP/04378/2020 and UIDB/04378/2020 of the Research Unit on Applied Molecular Biosciences \u2013 UCIBIO, and LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy - i4HB. ISP and MPL were supported by PhD fellowships SFRH/BD/129756/2017 and SFRH/BD/144284/2019, respectively, also funded by FCT. Open access funding provided by FCT|FCCN (b-on) Publisher Copyright: © The Author(s) 2024.
publishDate 2024
dc.date.none.fl_str_mv 2024-10-10T22:41:15Z
2024-12
2024-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/173345
url http://hdl.handle.net/10362/173345
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Pereira, I. S., da Cunha, M., Leal, I. P., Luís, M. P., Gonçalves, P., Gonçalves, C., & Mota, L. J. (2024). Identification of homologs of the Chlamydia trachomatis effector CteG reveals a family of Chlamydiaceae type III secreted proteins that can be delivered into host cells. Medical Microbiology and Immunology, 213(1), Article 15. Advance online publication. https://doi.org/10.1007/s00430-024-00798-9
0300-8584
PURE: 100978246
https://doi.org/10.1007/s00430-024-00798-9
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 19
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instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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