The Chlamydia trachomatis IncM Protein Interferes with Host Cell Cytokinesis, Centrosome Positioning, and Golgi Distribution and Contributes to the Stability of the Pathogen-Containing Vacuole

Bibliographic Details
Main Author: Luís, Maria Pequito
Publication Date: 2023
Other Authors: Pereira, Inês Serrano, Bugalhão, Joana N., Simões, Catarina N., Mota, Cristiano, Romão, Maria João, Mota, Luís Jaime
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10362/151622
Summary: We thank Irina Franco for critical reading of the manuscript and Agathe Subtil, Guangming Zhong, Ian Clarke, and Joao Paulo Gomes for gifts of antibodies and chlamydial genomic DNA. This work was supported by Fundacao para a Ciencia e a Tecnologia (FCT) in the scope of project UIDB/ 04378/2020 of the Research Unit on Applied Molecular Biosciences, UCIBIO, and LA/P/0140/ 2020 of the Associate Laboratory Institute for Health and Bioeconomy, i4HB. M.P.L. and I.S.P. were supported by PhD fellowships. J.N.B. was supported within the scope of the PhD program Molecular Biosciences (PD/00133/2012), also funded by FCT.
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spelling The Chlamydia trachomatis IncM Protein Interferes with Host Cell Cytokinesis, Centrosome Positioning, and Golgi Distribution and Contributes to the Stability of the Pathogen-Containing VacuoleImmunologyInfectious DiseasescentrosomesChlamydiabacterial pathogenesisWe thank Irina Franco for critical reading of the manuscript and Agathe Subtil, Guangming Zhong, Ian Clarke, and Joao Paulo Gomes for gifts of antibodies and chlamydial genomic DNA. This work was supported by Fundacao para a Ciencia e a Tecnologia (FCT) in the scope of project UIDB/ 04378/2020 of the Research Unit on Applied Molecular Biosciences, UCIBIO, and LA/P/0140/ 2020 of the Associate Laboratory Institute for Health and Bioeconomy, i4HB. M.P.L. and I.S.P. were supported by PhD fellowships. J.N.B. was supported within the scope of the PhD program Molecular Biosciences (PD/00133/2012), also funded by FCT.Chlamydia trachomatis is an obligate intracellular bacterial pathogen that causes ocular and urogenital infections in humans. The ability of C. trachomatis to grow intracellularly in a pathogen-containing vacuole (known as an inclusion) depends on chlamydial effector proteins transported into the host cell by a type III secretion system. Among these effectors, several inclusion membrane proteins (Incs) insert in the vacuolar membrane. Here, we show that human cell lines infected by a C. trachomatis strain deficient for Inc CT288/CTL0540 (renamed IncM) displayed less multinucleation than when infected by IncM-producing strains (wild type or complemented). This indicated that IncM is involved in the ability of Chlamydia to inhibit host cell cytokinesis. The capacity of IncM to induce multinucleation in infected cells was shown to be conserved among its chlamydial homologues and appeared to require its two larger regions predicted to be exposed to the host cell cytosol. C. trachomatis-infected cells also displayed IncM-dependent defects in centrosome positioning, Golgi distribution around the inclusion, and morphology and stability of the inclusion. The altered morphology of inclusions containing IncM-deficient C. trachomatis was further affected by depolymerization of host cell microtubules. This was not observed after depolymerization of microfilaments, and inclusions containing wild-type C. trachomatis did not alter their morphology upon depolymerization of microtubules. Overall, these findings suggest that IncM may exert its effector function by acting directly or indirectly on host cell microtubules.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNLuís, Maria PequitoPereira, Inês SerranoBugalhão, Joana N.Simões, Catarina N.Mota, CristianoRomão, Maria JoãoMota, Luís Jaime2023-04-05T22:16:31Z2023-04-182023-04-18T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/151622eng0019-9567PURE: 55665266https://doi.org/10.1128/iai.00405-22info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-22T18:10:50Zoai:run.unl.pt:10362/151622Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T17:41:11.678467Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv The Chlamydia trachomatis IncM Protein Interferes with Host Cell Cytokinesis, Centrosome Positioning, and Golgi Distribution and Contributes to the Stability of the Pathogen-Containing Vacuole
title The Chlamydia trachomatis IncM Protein Interferes with Host Cell Cytokinesis, Centrosome Positioning, and Golgi Distribution and Contributes to the Stability of the Pathogen-Containing Vacuole
spellingShingle The Chlamydia trachomatis IncM Protein Interferes with Host Cell Cytokinesis, Centrosome Positioning, and Golgi Distribution and Contributes to the Stability of the Pathogen-Containing Vacuole
Luís, Maria Pequito
Immunology
Infectious Diseases
centrosomes
Chlamydia
bacterial pathogenesis
title_short The Chlamydia trachomatis IncM Protein Interferes with Host Cell Cytokinesis, Centrosome Positioning, and Golgi Distribution and Contributes to the Stability of the Pathogen-Containing Vacuole
title_full The Chlamydia trachomatis IncM Protein Interferes with Host Cell Cytokinesis, Centrosome Positioning, and Golgi Distribution and Contributes to the Stability of the Pathogen-Containing Vacuole
title_fullStr The Chlamydia trachomatis IncM Protein Interferes with Host Cell Cytokinesis, Centrosome Positioning, and Golgi Distribution and Contributes to the Stability of the Pathogen-Containing Vacuole
title_full_unstemmed The Chlamydia trachomatis IncM Protein Interferes with Host Cell Cytokinesis, Centrosome Positioning, and Golgi Distribution and Contributes to the Stability of the Pathogen-Containing Vacuole
title_sort The Chlamydia trachomatis IncM Protein Interferes with Host Cell Cytokinesis, Centrosome Positioning, and Golgi Distribution and Contributes to the Stability of the Pathogen-Containing Vacuole
author Luís, Maria Pequito
author_facet Luís, Maria Pequito
Pereira, Inês Serrano
Bugalhão, Joana N.
Simões, Catarina N.
Mota, Cristiano
Romão, Maria João
Mota, Luís Jaime
author_role author
author2 Pereira, Inês Serrano
Bugalhão, Joana N.
Simões, Catarina N.
Mota, Cristiano
Romão, Maria João
Mota, Luís Jaime
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv DCV - Departamento de Ciências da Vida
UCIBIO - Applied Molecular Biosciences Unit
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Luís, Maria Pequito
Pereira, Inês Serrano
Bugalhão, Joana N.
Simões, Catarina N.
Mota, Cristiano
Romão, Maria João
Mota, Luís Jaime
dc.subject.por.fl_str_mv Immunology
Infectious Diseases
centrosomes
Chlamydia
bacterial pathogenesis
topic Immunology
Infectious Diseases
centrosomes
Chlamydia
bacterial pathogenesis
description We thank Irina Franco for critical reading of the manuscript and Agathe Subtil, Guangming Zhong, Ian Clarke, and Joao Paulo Gomes for gifts of antibodies and chlamydial genomic DNA. This work was supported by Fundacao para a Ciencia e a Tecnologia (FCT) in the scope of project UIDB/ 04378/2020 of the Research Unit on Applied Molecular Biosciences, UCIBIO, and LA/P/0140/ 2020 of the Associate Laboratory Institute for Health and Bioeconomy, i4HB. M.P.L. and I.S.P. were supported by PhD fellowships. J.N.B. was supported within the scope of the PhD program Molecular Biosciences (PD/00133/2012), also funded by FCT.
publishDate 2023
dc.date.none.fl_str_mv 2023-04-05T22:16:31Z
2023-04-18
2023-04-18T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/151622
url http://hdl.handle.net/10362/151622
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0019-9567
PURE: 55665266
https://doi.org/10.1128/iai.00405-22
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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