Effective reduction of Salmonella Enteritidis in broiler chickens using the UPWr_S134 phage cocktail

Bibliographic Details
Main Author: Kuzminska-Bajor, Marta
Publication Date: 2023
Other Authors: Sliwka, Paulina, Korzeniowski, Pawel, Kuczkowski, Maciej, Sáez, David, Wozniak-Biel, Anna, Sliwinska, Emilia, Grzymajlo, Krzysztof
Format: Article
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: https://hdl.handle.net/1822/84484
Summary: The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.
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spelling Effective reduction of Salmonella Enteritidis in broiler chickens using the UPWr_S134 phage cocktailSalmonellaphage cocktailantibacterial agentpoultrybiocontrolThe raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.Salmonella is a poultry-associated pathogen that is considered one of the most important zoonotic bacterial agents of contaminated food of animal origin including poultry products. Many efforts are taken to eliminate it from the food chain, and phages are one of the most promising tools to control Salmonella in poultry production. We investigated the usefulness of the UPWr_S134 phage cocktail in reducing Salmonella in broiler chickens. For this purpose, we analyzed the survivability of phages in the harsh environment encountered in the chicken gastrointestinal tract, which has low pH, high temperatures, and digestive activity. Phages in the cocktail UPWr_S134 showed the ability to remain active after storage at temperatures ranging from 4 to 42°C, reflecting temperatures of storage conditions, broiler handling, and the chicken body, and exhibited robust pH stability. We found that although simulated gastric fluids (SGF) caused phage inactivation, the addition of feed to gastric juice allows maintenance of UPWr_S134 phage cocktail activity. Further, we analyzed UPWr_S134 phage cocktail anti-Salmonella activity in live animals such as mice and broilers. In an acute infection model in mice, the application of doses of 107 and 1014 PFU/ml UPWr_S134 phage cocktail resulted in delaying symptoms of intrinsic infection in all analyzed treatment schedules. In Salmonella-infected chickens orally treated with the UPWr_S134 phage cocktail the number of pathogens in internal organs in comparison to untreated birds was significantly lower. Therefore we concluded that the UPWr_S134 phage cocktail could be an effective tool against this pathogen in the poultry industry.This study was supported by the National Centre for Research and Development, LIDER program no. LIDER/378/L-6/14/NCBR/2015. The APC was co-financed by Wroclaw University of Environmental and Life Sciences and University of Wroclaw and Biotechnologists Student Science Club.info:eu-repo/semantics/publishedVersionFrontiers Media S.A.Universidade do MinhoKuzminska-Bajor, MartaSliwka, PaulinaKorzeniowski, PawelKuczkowski, MaciejSáez, DavidWozniak-Biel, AnnaSliwinska, EmiliaGrzymajlo, Krzysztof20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/84484engKuźmińska-Bajor M, Śliwka P, Korzeniowski P, Kuczkowski M, Moreno DS, Woźniak-Biel A, Śliwińska E and Grzymajło K (2023) Effective reduction of Salmonella Enteritidis in broiler chickens using the UPWr_S134 phage cocktail. Front. Microbiol. 14:1136261. doi: 10.3389/fmicb.2023.11362611664-302X10.3389/fmicb.2023.11362611136261https://www.frontiersin.org/articles/10.3389/fmicb.2023.1136261/fullinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T06:36:20Zoai:repositorium.sdum.uminho.pt:1822/84484Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T15:58:26.883093Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Effective reduction of Salmonella Enteritidis in broiler chickens using the UPWr_S134 phage cocktail
title Effective reduction of Salmonella Enteritidis in broiler chickens using the UPWr_S134 phage cocktail
spellingShingle Effective reduction of Salmonella Enteritidis in broiler chickens using the UPWr_S134 phage cocktail
Kuzminska-Bajor, Marta
Salmonella
phage cocktail
antibacterial agent
poultry
biocontrol
title_short Effective reduction of Salmonella Enteritidis in broiler chickens using the UPWr_S134 phage cocktail
title_full Effective reduction of Salmonella Enteritidis in broiler chickens using the UPWr_S134 phage cocktail
title_fullStr Effective reduction of Salmonella Enteritidis in broiler chickens using the UPWr_S134 phage cocktail
title_full_unstemmed Effective reduction of Salmonella Enteritidis in broiler chickens using the UPWr_S134 phage cocktail
title_sort Effective reduction of Salmonella Enteritidis in broiler chickens using the UPWr_S134 phage cocktail
author Kuzminska-Bajor, Marta
author_facet Kuzminska-Bajor, Marta
Sliwka, Paulina
Korzeniowski, Pawel
Kuczkowski, Maciej
Sáez, David
Wozniak-Biel, Anna
Sliwinska, Emilia
Grzymajlo, Krzysztof
author_role author
author2 Sliwka, Paulina
Korzeniowski, Pawel
Kuczkowski, Maciej
Sáez, David
Wozniak-Biel, Anna
Sliwinska, Emilia
Grzymajlo, Krzysztof
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Kuzminska-Bajor, Marta
Sliwka, Paulina
Korzeniowski, Pawel
Kuczkowski, Maciej
Sáez, David
Wozniak-Biel, Anna
Sliwinska, Emilia
Grzymajlo, Krzysztof
dc.subject.por.fl_str_mv Salmonella
phage cocktail
antibacterial agent
poultry
biocontrol
topic Salmonella
phage cocktail
antibacterial agent
poultry
biocontrol
description The raw data supporting the conclusions of this article will be made available by the authors, without undue reservation.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/84484
url https://hdl.handle.net/1822/84484
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Kuźmińska-Bajor M, Śliwka P, Korzeniowski P, Kuczkowski M, Moreno DS, Woźniak-Biel A, Śliwińska E and Grzymajło K (2023) Effective reduction of Salmonella Enteritidis in broiler chickens using the UPWr_S134 phage cocktail. Front. Microbiol. 14:1136261. doi: 10.3389/fmicb.2023.1136261
1664-302X
10.3389/fmicb.2023.1136261
1136261
https://www.frontiersin.org/articles/10.3389/fmicb.2023.1136261/full
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
_version_ 1833595657870376960

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