Cell-extrallular matrix interactions during early paraxial mesoderm development

Bibliographic Details
Main Author: Lopes, Catarina Teixeira
Publication Date: 2008
Format: Master thesis
Language: por
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/1222
Summary: Tese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2007, Universidade de Lisboa, Faculdade de Ciências
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spelling Cell-extrallular matrix interactions during early paraxial mesoderm developmentEmbriologiaBiologia evolutivaTeses de mestradoTese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2007, Universidade de Lisboa, Faculdade de CiênciasFibronectin is an abundant glycoprotein of the extracellular matrix, which has been shown to play a role in a variety of functions such as cell proliferation, survival, migration and differentiation. Fibronectin is assembled into a fibrillar matrix through a cell-mediated process involving their heterodimeric membrane receptors, integrins. Recently, the work of our group has evidenced the importance of fibronectin in somitogenesis and how the ectoderm and mesoderm collaborate in the construction of the fibronectin matrix of presomitic mesoderm (Rifes et al., 2007). In the present work, we further characterised the expression patterns of fibronectin (Fn1), integrin α5 (Itga5), the main sub-unit involved in fibronectin assembly, and integrin α4 (Itga4), during chick embryonic development. Fn1 transcripts are strongly expressed in most epithelia, mainly in the ectoderm in all stages studied. Itga5 is initially expressed in the primitive streak and node and it is later found in the ingressing mesoderm and many of its derivates, especially the heart and vascular primordia. Itga4 transcripts are also detected in the node and mesoderm, but later it is expressed in the dorsal neural tube and somite derivates. Finally, we also established an in vitro culture system of presomitic mesoderm cells by using different enzymes and substrata. Immunofluorescence labelling of fibronectin shows that isolated PSM cells in culture can assembly a fibronectin matrix, even when it was previously removed by dispase. In the tested culture conditions, these cells remained in a non-proliferative mesenchymal-like state, expressing very low levels of both N-cadherin and ß-catenin. These data suggest that fibronectin is not only an important component of the extracellular matrix in vivo and in vitro, but also that its presence in various tissues during the early stages of chick development is imperativeRodrigues, Maria GabrielaRepositório da Universidade de LisboaLopes, Catarina Teixeira2010-07-27T08:55:35Z20082008-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdftext/xmlhttp://hdl.handle.net/10451/1222porhttp://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000559481info:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T12:38:20Zoai:repositorio.ulisboa.pt:10451/1222Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T02:24:48.792212Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Cell-extrallular matrix interactions during early paraxial mesoderm development
title Cell-extrallular matrix interactions during early paraxial mesoderm development
spellingShingle Cell-extrallular matrix interactions during early paraxial mesoderm development
Lopes, Catarina Teixeira
Embriologia
Biologia evolutiva
Teses de mestrado
title_short Cell-extrallular matrix interactions during early paraxial mesoderm development
title_full Cell-extrallular matrix interactions during early paraxial mesoderm development
title_fullStr Cell-extrallular matrix interactions during early paraxial mesoderm development
title_full_unstemmed Cell-extrallular matrix interactions during early paraxial mesoderm development
title_sort Cell-extrallular matrix interactions during early paraxial mesoderm development
author Lopes, Catarina Teixeira
author_facet Lopes, Catarina Teixeira
author_role author
dc.contributor.none.fl_str_mv Rodrigues, Maria Gabriela
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Lopes, Catarina Teixeira
dc.subject.por.fl_str_mv Embriologia
Biologia evolutiva
Teses de mestrado
topic Embriologia
Biologia evolutiva
Teses de mestrado
description Tese de mestrado, Biologia (Biologia Evolutiva e do Desenvolvimento), 2007, Universidade de Lisboa, Faculdade de Ciências
publishDate 2008
dc.date.none.fl_str_mv 2008
2008-01-01T00:00:00Z
2010-07-27T08:55:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/1222
url http://hdl.handle.net/10451/1222
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv http://catalogo.ul.pt/F/?func=item-global&doc_library=ULB01&type=03&doc_number=000559481
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
text/xml
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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