Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems

Detalhes bibliográficos
Autor(a) principal: Cunha, Sara C.
Data de Publicação: 2006
Outros Autores: Amaral, Joana S., Fernandes, J.O., Oliveira, Beatriz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/10198/1483
Resumo: Three different HPLC detection systems were compared for the determination of tocopherols and tocotrienols in olive oil: fluorescence and diode array connected in series, ultraviolet, and evaporative light scattering. The best results were obtained with the fluorescence detector, which was successfully applied in the quantification of tocopherols and tocotrienols in 18 samples of Portuguese olive oils. To support the validity of the method, the parameters evaluated were linearity, detection limits, repeatability, and recovery. All of the studied samples showed similar qualitative profiles with six identified compounds: R-T, -T, γ-T, δ-T, R-T3, and γ-T3. R-Tocopherol (R-T) was the main vitamin E isomer in all samples ranging from 93 to 260 mg/kg. The total tocopherols and tocotrienols ranged from 100 to 270 mg/kg. Geographic origin did not seem to influence the tocopherol and tocotrienol composition of the olive oils under evaluation.
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spelling Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systemsTocopherolsTocotrienolsOlive oilNP-HPLCThree different HPLC detection systems were compared for the determination of tocopherols and tocotrienols in olive oil: fluorescence and diode array connected in series, ultraviolet, and evaporative light scattering. The best results were obtained with the fluorescence detector, which was successfully applied in the quantification of tocopherols and tocotrienols in 18 samples of Portuguese olive oils. To support the validity of the method, the parameters evaluated were linearity, detection limits, repeatability, and recovery. All of the studied samples showed similar qualitative profiles with six identified compounds: R-T, -T, γ-T, δ-T, R-T3, and γ-T3. R-Tocopherol (R-T) was the main vitamin E isomer in all samples ranging from 93 to 260 mg/kg. The total tocopherols and tocotrienols ranged from 100 to 270 mg/kg. Geographic origin did not seem to influence the tocopherol and tocotrienol composition of the olive oils under evaluation.ACSBiblioteca Digital do IPBCunha, Sara C.Amaral, Joana S.Fernandes, J.O.Oliveira, Beatriz2010-01-26T22:00:40Z20062006-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/1483engCunha, S.C.; Amaral, J.S.; Fernandes, J.O.; Oliveira, M.B.P.P. (2006). Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems. Journal of Agricultural and Food Chemistry. ISSN 0021-8561. 54:9, p.3351–33560021-856110.1021/jf053102ninfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-02-25T11:55:13Zoai:bibliotecadigital.ipb.pt:10198/1483Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T11:16:45.726472Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems
title Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems
spellingShingle Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems
Cunha, Sara C.
Tocopherols
Tocotrienols
Olive oil
NP-HPLC
title_short Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems
title_full Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems
title_fullStr Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems
title_full_unstemmed Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems
title_sort Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems
author Cunha, Sara C.
author_facet Cunha, Sara C.
Amaral, Joana S.
Fernandes, J.O.
Oliveira, Beatriz
author_role author
author2 Amaral, Joana S.
Fernandes, J.O.
Oliveira, Beatriz
author2_role author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Cunha, Sara C.
Amaral, Joana S.
Fernandes, J.O.
Oliveira, Beatriz
dc.subject.por.fl_str_mv Tocopherols
Tocotrienols
Olive oil
NP-HPLC
topic Tocopherols
Tocotrienols
Olive oil
NP-HPLC
description Three different HPLC detection systems were compared for the determination of tocopherols and tocotrienols in olive oil: fluorescence and diode array connected in series, ultraviolet, and evaporative light scattering. The best results were obtained with the fluorescence detector, which was successfully applied in the quantification of tocopherols and tocotrienols in 18 samples of Portuguese olive oils. To support the validity of the method, the parameters evaluated were linearity, detection limits, repeatability, and recovery. All of the studied samples showed similar qualitative profiles with six identified compounds: R-T, -T, γ-T, δ-T, R-T3, and γ-T3. R-Tocopherol (R-T) was the main vitamin E isomer in all samples ranging from 93 to 260 mg/kg. The total tocopherols and tocotrienols ranged from 100 to 270 mg/kg. Geographic origin did not seem to influence the tocopherol and tocotrienol composition of the olive oils under evaluation.
publishDate 2006
dc.date.none.fl_str_mv 2006
2006-01-01T00:00:00Z
2010-01-26T22:00:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/1483
url http://hdl.handle.net/10198/1483
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cunha, S.C.; Amaral, J.S.; Fernandes, J.O.; Oliveira, M.B.P.P. (2006). Quantification of tocopherols and tocotrienols in Portuguese olive oils using HPLC with three different detection systems. Journal of Agricultural and Food Chemistry. ISSN 0021-8561. 54:9, p.3351–3356
0021-8561
10.1021/jf053102n
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ACS
publisher.none.fl_str_mv ACS
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
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reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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