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Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44

Detalhes bibliográficos
Autor(a) principal: Amorim, Sara
Data de Publicação: 2020
Outros Autores: Pashkuleva, I., Reis, Celso A., Reis, R. L., Pires, R. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Texto Completo: http://hdl.handle.net/1822/66347
Resumo: We report on the development of layer-by-layer (LbL) constructs whose viscoelastic properties and bioactivity can be finely tuned by using polyanions of different size and/or crosslinking. As a polyanion we used hyaluronic acid (HA) â a multi-signaling biomolecule whose bioactivity depends on its molecular weight. We investigated the interplay between the mechanical properties of the LbL systems built using HA of different sizes and the specific HA-mediated biochemical interactions. We characterized the assembled materials and their interactions with CD44, the main HA receptor, by Quartz Crystal Microbalance with Dissipation (QCM-D), Surface Plasmon Resonance (SPR) and Atomic Force Microscopy (AFM). We observed that the presence of CD44 resulted in the disruption of the non-crosslinked multilayers, while crosslinked films remain stable and bind CD44 in a HA molecular weight and charge specific fashion.
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spelling Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44BioactivityCD44Hyaluronic acidScience & TechnologyWe report on the development of layer-by-layer (LbL) constructs whose viscoelastic properties and bioactivity can be finely tuned by using polyanions of different size and/or crosslinking. As a polyanion we used hyaluronic acid (HA) â a multi-signaling biomolecule whose bioactivity depends on its molecular weight. We investigated the interplay between the mechanical properties of the LbL systems built using HA of different sizes and the specific HA-mediated biochemical interactions. We characterized the assembled materials and their interactions with CD44, the main HA receptor, by Quartz Crystal Microbalance with Dissipation (QCM-D), Surface Plasmon Resonance (SPR) and Atomic Force Microscopy (AFM). We observed that the presence of CD44 resulted in the disruption of the non-crosslinked multilayers, while crosslinked films remain stable and bind CD44 in a HA molecular weight and charge specific fashion.The authors acknowledge the financial support from the European Commission’s H2020 programme, under grant agreements H2020- WIDESPREAD-2014-668983-FORECAST and H2020-WIDESPREAD01-2016-2017-739572-THE DISCOVERIES CTR. S. A. acknowledges the Portuguese Foundation for Science and Technology (FCT) for her PhD grant (SFRH/BD/112075/2015)Royal Society of ChemistryUniversidade do MinhoAmorim, SaraPashkuleva, I.Reis, Celso A.Reis, R. L.Pires, R. A.2020-032020-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/66347engAmorim S., Pashkuleva I., Reis C. A., Reis R. L., Pires R. A. Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44, Journal Of Materials Chemistry B, Vol. 17, doi:10.1039/d0tb00407c, 20202050-750X2050-751810.1039/d0tb00407c32222753https://pubs.rsc.org/en/content/articlelanding/2020/tb/d0tb00407c/unauth#!divAbstractinfo:eu-repo/semantics/openAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2024-05-11T04:10:45Zoai:repositorium.sdum.uminho.pt:1822/66347Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-28T14:41:42.032070Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44
title Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44
spellingShingle Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44
Amorim, Sara
Bioactivity
CD44
Hyaluronic acid
Science & Technology
title_short Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44
title_full Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44
title_fullStr Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44
title_full_unstemmed Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44
title_sort Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44
author Amorim, Sara
author_facet Amorim, Sara
Pashkuleva, I.
Reis, Celso A.
Reis, R. L.
Pires, R. A.
author_role author
author2 Pashkuleva, I.
Reis, Celso A.
Reis, R. L.
Pires, R. A.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Amorim, Sara
Pashkuleva, I.
Reis, Celso A.
Reis, R. L.
Pires, R. A.
dc.subject.por.fl_str_mv Bioactivity
CD44
Hyaluronic acid
Science & Technology
topic Bioactivity
CD44
Hyaluronic acid
Science & Technology
description We report on the development of layer-by-layer (LbL) constructs whose viscoelastic properties and bioactivity can be finely tuned by using polyanions of different size and/or crosslinking. As a polyanion we used hyaluronic acid (HA) â a multi-signaling biomolecule whose bioactivity depends on its molecular weight. We investigated the interplay between the mechanical properties of the LbL systems built using HA of different sizes and the specific HA-mediated biochemical interactions. We characterized the assembled materials and their interactions with CD44, the main HA receptor, by Quartz Crystal Microbalance with Dissipation (QCM-D), Surface Plasmon Resonance (SPR) and Atomic Force Microscopy (AFM). We observed that the presence of CD44 resulted in the disruption of the non-crosslinked multilayers, while crosslinked films remain stable and bind CD44 in a HA molecular weight and charge specific fashion.
publishDate 2020
dc.date.none.fl_str_mv 2020-03
2020-03-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/66347
url http://hdl.handle.net/1822/66347
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Amorim S., Pashkuleva I., Reis C. A., Reis R. L., Pires R. A. Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44, Journal Of Materials Chemistry B, Vol. 17, doi:10.1039/d0tb00407c, 2020
2050-750X
2050-7518
10.1039/d0tb00407c
32222753
https://pubs.rsc.org/en/content/articlelanding/2020/tb/d0tb00407c/unauth#!divAbstract
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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