Rhipicephalus sanguineus (Latreille, 1806) an important pathogen tick vector in Portugal: a metagenomic approach

Bibliographic Details
Main Author: Vieira, Mariana Esteves
Publication Date: 2023
Format: Master thesis
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10451/64122
Summary: Tese de mestrado, Biologia Humana e Ambiente, 2023, Universidade de Lisboa, Faculdade de Ciências
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spelling Rhipicephalus sanguineus (Latreille, 1806) an important pathogen tick vector in Portugal: a metagenomic approachRhipicephalus sanguineuspatogéniosCOIsequenciação metagenómica por ShotgunPortugal ContinentalTeses de mestrado - 2024Departamento de Biologia AnimalTese de mestrado, Biologia Humana e Ambiente, 2023, Universidade de Lisboa, Faculdade de CiênciasTicks are hematophagous arthropods represented by approximately 900 species, divided into three families: Argasidae (soft-bodied ticks), Ixodidae (hard-bodied ticks) and Nuttalliellidae (intermediate features). Rhipicephalus sanguineus, belonging to the Ixodidae family, is the dominant species in mainland Portugal. This species is the vector of several pathogens, many of them emerging in our country. Most of them belong to Rickettsia (Rickettsia massiliae, Ri. conorii), Coxiella, Anaplasma, Babesia and Ehrlichia. It is therefore necessary to know the true epidemiological situation in mainland Portugal, which tick species are present and which potential pathogens they carry. Thus, this work aims to identify which tick species are present in dogs and cats from mainland Portugal and which pathogens are found in Rhipicephalus sanguineus ticks, in particular. Ticks were morphological identified based on diagnostic characteristics, and when in doubt, we resorted to molecular identification through Cytochrome C Oxidase subunit I (COI) and 16S gene. Next, the presence of potential pathogens from Rickettsia, Anaplasma/Ehrlichia, and Babesia/Hepatozoon genera was obtained using specific molecular markers by RT-PCR. A Shotgun metagenomic sequencing analysis was also carried out with Kraken 2 and Krona, to obtain more detailed information on the set of pathogens present in R. sanguineus samples, one for each studied region (North, Centre and South). The results showed a more evident presence of Rickettsia genus, namely Rickettsia massiliae, and, for the first time, the bacterium Rickettsia amblyommatis and the endosymbiont Candidatus Coxiella mudrowiae were identified in mainland Portugal. The presence of Klebsiella pneumoniae and Staphylococcus pseudointermedius was also detected in Rhipicephalus sanguineus. To conclude, the data obtained from this study contributed to a better understanding of ticks’ distribution in Portugal, the bacterial communities hosted by Rhipicephalus sanguineus and allowed us to see what other pathogens not frequently associated with this species are circulating in Portuguese territory.Dias, Deodália Maria Antunes, 1952-Rosa, FernandaRepositório da Universidade de LisboaVieira, Mariana Esteves202420232026-11-24T00:00:00Z2024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10451/64122enginfo:eu-repo/semantics/embargoedAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T15:14:05Zoai:repositorio.ulisboa.pt:10451/64122Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T03:37:35.831856Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Rhipicephalus sanguineus (Latreille, 1806) an important pathogen tick vector in Portugal: a metagenomic approach
title Rhipicephalus sanguineus (Latreille, 1806) an important pathogen tick vector in Portugal: a metagenomic approach
spellingShingle Rhipicephalus sanguineus (Latreille, 1806) an important pathogen tick vector in Portugal: a metagenomic approach
Vieira, Mariana Esteves
Rhipicephalus sanguineus
patogénios
COI
sequenciação metagenómica por Shotgun
Portugal Continental
Teses de mestrado - 2024
Departamento de Biologia Animal
title_short Rhipicephalus sanguineus (Latreille, 1806) an important pathogen tick vector in Portugal: a metagenomic approach
title_full Rhipicephalus sanguineus (Latreille, 1806) an important pathogen tick vector in Portugal: a metagenomic approach
title_fullStr Rhipicephalus sanguineus (Latreille, 1806) an important pathogen tick vector in Portugal: a metagenomic approach
title_full_unstemmed Rhipicephalus sanguineus (Latreille, 1806) an important pathogen tick vector in Portugal: a metagenomic approach
title_sort Rhipicephalus sanguineus (Latreille, 1806) an important pathogen tick vector in Portugal: a metagenomic approach
author Vieira, Mariana Esteves
author_facet Vieira, Mariana Esteves
author_role author
dc.contributor.none.fl_str_mv Dias, Deodália Maria Antunes, 1952-
Rosa, Fernanda
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Vieira, Mariana Esteves
dc.subject.por.fl_str_mv Rhipicephalus sanguineus
patogénios
COI
sequenciação metagenómica por Shotgun
Portugal Continental
Teses de mestrado - 2024
Departamento de Biologia Animal
topic Rhipicephalus sanguineus
patogénios
COI
sequenciação metagenómica por Shotgun
Portugal Continental
Teses de mestrado - 2024
Departamento de Biologia Animal
description Tese de mestrado, Biologia Humana e Ambiente, 2023, Universidade de Lisboa, Faculdade de Ciências
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024-01-01T00:00:00Z
2026-11-24T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/64122
url http://hdl.handle.net/10451/64122
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron_str RCAAP
institution RCAAP
reponame_str Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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