Comparative genome analysis of morphologically distinct populations of Rhipicephalus sanguineus (Latreille, 1806)

Bibliographic Details
Main Author: Carvalho, Mário André Barbosa de
Publication Date: 2024
Format: Master thesis
Language: eng
Source: Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
Download full: http://hdl.handle.net/10400.5/97158
Summary: Tese de mestrado, Biologia Humana e Ambiente, 2024, Universidade de Lisboa, Faculdade de Ciências
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spelling Comparative genome analysis of morphologically distinct populations of Rhipicephalus sanguineus (Latreille, 1806)Rhipicephalus sanguineusvariabilidade morfológicavariabilidade genéticapopulações de carraçasPenínsula IbéricaTeses de mestrado - 2024Departamento de Biologia AnimalTese de mestrado, Biologia Humana e Ambiente, 2024, Universidade de Lisboa, Faculdade de CiênciasTicks are arthropod hematophagous parasites that can transmit pathogens to animals, including humans. So, the accurate discrimination of species is important for understanding epidemiological context of the pathogens they might transmit. Closely related tick species, and different populations within a tick species, can evidence diverse ability to transmit pathogens. The main objective of the present study was to know if the Rhipicephalus sanguineus specimens of different populations collected from vegetation with two distinct morphologies may be distinguished by the mitochondrial genes COI and rRNA 16S, and the nuclear ribosomal internal transcribed spacer 2 (ITS2). A total of 75 specimens were collected in four areas with two set of morphologies, which were morphometrically characterised based on female genitalia and male and female spiracles. DNA was successfully extracted from 38 specimens and 56 products of PCR were obtained for COI, 16S and ITS2 markers. Sequences obtained were subjected to Blast in the NCBI databases, p–distance estimations and Maximum Likelihood (ML) phylogenetic reconstructions. The morphologies identified, designated as morphology A and morphology B, evidenced significant differences (p–value 0.01) in all studied traits of female and male. While 16S and ITS2 markers did not fully contribute for accurate molecular distinctions, COI sequences from individuals of both morphologies evidenced values of p-distance of 1%, in strict correspondence to four polymorphic positions, also segregating in distinct well-supported clades in the ML phylogenetic tree. For the first time, a clear molecular distinction between the two morphologies was identified but, unless further studies prove specific discrimination, most likely they still are members from the same species. However, few sequences were analysed for 16S and ITS2 markers. Further research on nuclear markers and whole genome sequencing are required to fully elucidate the level of genomic discrimination between these morphotypes, like between mitochondrial and nuclear genes.Caeiro, Maria Filomena TrabuchoRosa, Fernanda Henriques de JesusRepositório da Universidade de LisboaCarvalho, Mário André Barbosa de202420242026-10-30T00:00:00Z2024-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.5/97158enginfo:eu-repo/semantics/embargoedAccessreponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiainstacron:RCAAP2025-03-17T16:31:43Zoai:repositorio.ulisboa.pt:10400.5/97158Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireinfo@rcaap.ptopendoar:https://opendoar.ac.uk/repository/71602025-05-29T04:18:17.772882Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologiafalse
dc.title.none.fl_str_mv Comparative genome analysis of morphologically distinct populations of Rhipicephalus sanguineus (Latreille, 1806)
title Comparative genome analysis of morphologically distinct populations of Rhipicephalus sanguineus (Latreille, 1806)
spellingShingle Comparative genome analysis of morphologically distinct populations of Rhipicephalus sanguineus (Latreille, 1806)
Carvalho, Mário André Barbosa de
Rhipicephalus sanguineus
variabilidade morfológica
variabilidade genética
populações de carraças
Península Ibérica
Teses de mestrado - 2024
Departamento de Biologia Animal
title_short Comparative genome analysis of morphologically distinct populations of Rhipicephalus sanguineus (Latreille, 1806)
title_full Comparative genome analysis of morphologically distinct populations of Rhipicephalus sanguineus (Latreille, 1806)
title_fullStr Comparative genome analysis of morphologically distinct populations of Rhipicephalus sanguineus (Latreille, 1806)
title_full_unstemmed Comparative genome analysis of morphologically distinct populations of Rhipicephalus sanguineus (Latreille, 1806)
title_sort Comparative genome analysis of morphologically distinct populations of Rhipicephalus sanguineus (Latreille, 1806)
author Carvalho, Mário André Barbosa de
author_facet Carvalho, Mário André Barbosa de
author_role author
dc.contributor.none.fl_str_mv Caeiro, Maria Filomena Trabucho
Rosa, Fernanda Henriques de Jesus
Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Carvalho, Mário André Barbosa de
dc.subject.por.fl_str_mv Rhipicephalus sanguineus
variabilidade morfológica
variabilidade genética
populações de carraças
Península Ibérica
Teses de mestrado - 2024
Departamento de Biologia Animal
topic Rhipicephalus sanguineus
variabilidade morfológica
variabilidade genética
populações de carraças
Península Ibérica
Teses de mestrado - 2024
Departamento de Biologia Animal
description Tese de mestrado, Biologia Humana e Ambiente, 2024, Universidade de Lisboa, Faculdade de Ciências
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024-01-01T00:00:00Z
2026-10-30T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/97158
url http://hdl.handle.net/10400.5/97158
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
instname:FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
instacron:RCAAP
instname_str FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
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collection Repositórios Científicos de Acesso Aberto de Portugal (RCAAP)
repository.name.fl_str_mv Repositórios Científicos de Acesso Aberto de Portugal (RCAAP) - FCCN, serviços digitais da FCT – Fundação para a Ciência e a Tecnologia
repository.mail.fl_str_mv info@rcaap.pt
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