B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis
| Main Author: | |
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| Publication Date: | 2014 |
| Other Authors: | , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1111/pim.12111 |
| Download full: | https://arca.fiocruz.br/handle/icict/24882 |
Summary: | CARVALHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. |
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Assis, Luciana Moura deSousa, J. R.Pinto, N. F. S.Silva, A. A.Vaz, Antonio Fernando de MeloAndrade, Paulo PaesCarvalho, Edgar Marcelino deMelo, Marcia Almeida de2018-02-20T14:18:03Z2018-02-20T14:18:03Z2014ASSIS, L. M. et al. B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis. Parasite Immunology, v. 36, p. 313–323, 2014.0141-9838https://arca.fiocruz.br/handle/icict/2488210.1111/pim.12111CARVALHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.National Institute of Science and Technology of Tropical Diseases (Instituto Nacional de Ci^encia e Tecnologia de Doenc as Tropicais – INCT-DT), MCT/CNPq,Federal University of Bahia. University Hospital Complex Professor Edgard Santos. Program in Medicine and Health. Salvador, BA, Brazil / Federal University of Campina Grande. Academic Nursing Unit. Cajazeiras, CG, BrazilFaculdade Santa Maria. Santa Maria College. Cajazeiras, CG, BrazilFederal University of Campina Grande. Veterinary Medicine Graduate. Program in Veterinary Medicine Academic Unit. Patos, CG, BrasilFederal University of Campina Grande. Academic Unit of Exact and Natural Sciences. Cajazeiras, CG, BrazilFederal University of Campina Grande. Academic Unit Veterinary Medicine. Patos, CG, BrazilFederal University of Pernambuco. Department of Genetics. Recife, PE, BrazilFederal University of Bahia. Professor Edgard Santos University Hospital Complex. Graduate Program in Medicine and Health, Immunology Service. Salvador, BA, Brazil / National Institute of Science and Technology of Tropical Diseases. Salvador, BA, BrazilFederal University of Campina Grande. Academic Unit Veterinary Medicine. Patos, CG, BrazilSerodiagnosis of visceral leishmaniasis is often hindered by cross-reactions to other parasitic diseases. Identifying specific B-cell epitopes in proteins is therefore important for immunodiagnostics, as well as for disease control by vaccines. This study aimed to identify linear and conformational B-cell epitopes and to evaluate the secondary structure of antigen proteins in Leishmania infantum using in silico analysis. Linear epitopes were predicted using the Immune Epitope Database and Analysis Resource (IEDB), BepiPred and BcePred programs. The conformational B-cell epitopes were identified using the CBTOPE server. The combination of the predictions using IEDB, BepiPred and BcePred generated 148 linear epitopes from the calpain-like cysteine peptidase (CP), thiol-dependent reductase 1 (TDR1) and HSP70 proteins. In total, 164 conformational epitopes were predicted, mostly located in the linear epitope region. The predicted epitopes are located in α helix and random coil regions in the thiol-dependent reductase 1 and HSP70 proteins. New linear and conformational B-cell epitopes of L. infantum proteins were identified in silico, and the prediction using various programs ensures greater accuracy of the results, as suggested by confirmation of previously identified HSP70 epitopes.engWilleyBioinformáticaDiagnósticoKala-azarBiologia sintéticaVacinaBioinformaticsDiagnosisKala-azarSynthetic biologyVaccine03 Saúde e Bem-EstarB-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/0d22c213-4c66-48b3-b0fe-4746abc0f932/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALAssis LM B- cell epitopes of antigenic proteins....pdfAssis LM B- cell epitopes of antigenic proteins....pdfapplication/pdf1586892https://arca.fiocruz.br/bitstreams/58f5c0d0-b15e-4be6-b035-889f7587c4fc/download2bfc64c83fbd9d1c332850d885804695MD52trueAnonymousREADTEXTAssis LM B- cell epitopes of antigenic proteins....pdf.txtAssis LM B- cell epitopes of antigenic proteins....pdf.txtExtracted texttext/plain39448https://arca.fiocruz.br/bitstreams/a63335dd-019d-4562-9484-46d2f8a00bbe/download3d6b7501c389feb1f38ba15e8c1ecc59MD55falseAnonymousREADTHUMBNAILAssis LM B- cell epitopes of antigenic proteins....pdf.jpgAssis LM B- cell epitopes of antigenic proteins....pdf.jpgGenerated Thumbnailimage/jpeg5820https://arca.fiocruz.br/bitstreams/ac426fa7-c171-4f1a-b1b0-2ebe72b3c4f5/download18cea52e84f38f2a3102240f23b8bce1MD56falseAnonymousREADicict/248822025-08-03 15:46:37.478open.accessoai:arca.fiocruz.br:icict/24882https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-08-03T18:46:37Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.none.fl_str_mv |
B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis |
| title |
B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis |
| spellingShingle |
B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis Assis, Luciana Moura de Bioinformática Diagnóstico Kala-azar Biologia sintética Vacina Bioinformatics Diagnosis Kala-azar Synthetic biology Vaccine 03 Saúde e Bem-Estar |
| title_short |
B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis |
| title_full |
B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis |
| title_fullStr |
B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis |
| title_full_unstemmed |
B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis |
| title_sort |
B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis |
| author |
Assis, Luciana Moura de |
| author_facet |
Assis, Luciana Moura de Sousa, J. R. Pinto, N. F. S. Silva, A. A. Vaz, Antonio Fernando de Melo Andrade, Paulo Paes Carvalho, Edgar Marcelino de Melo, Marcia Almeida de |
| author_role |
author |
| author2 |
Sousa, J. R. Pinto, N. F. S. Silva, A. A. Vaz, Antonio Fernando de Melo Andrade, Paulo Paes Carvalho, Edgar Marcelino de Melo, Marcia Almeida de |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Assis, Luciana Moura de Sousa, J. R. Pinto, N. F. S. Silva, A. A. Vaz, Antonio Fernando de Melo Andrade, Paulo Paes Carvalho, Edgar Marcelino de Melo, Marcia Almeida de |
| dc.subject.other.none.fl_str_mv |
Bioinformática Diagnóstico Kala-azar Biologia sintética Vacina |
| topic |
Bioinformática Diagnóstico Kala-azar Biologia sintética Vacina Bioinformatics Diagnosis Kala-azar Synthetic biology Vaccine 03 Saúde e Bem-Estar |
| dc.subject.en.none.fl_str_mv |
Bioinformatics Diagnosis Kala-azar Synthetic biology Vaccine |
| dc.subject.ods.none.fl_str_mv |
03 Saúde e Bem-Estar |
| description |
CARVALHO, Edgar Marcelino de. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”. |
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2014 |
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2014 |
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2018-02-20T14:18:03Z |
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2018-02-20T14:18:03Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
ASSIS, L. M. et al. B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis. Parasite Immunology, v. 36, p. 313–323, 2014. |
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https://arca.fiocruz.br/handle/icict/24882 |
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0141-9838 |
| dc.identifier.doi.none.fl_str_mv |
10.1111/pim.12111 |
| identifier_str_mv |
ASSIS, L. M. et al. B-cell epitopes of antigenic proteins in Leishmania infantum: an in silico analysis. Parasite Immunology, v. 36, p. 313–323, 2014. 0141-9838 10.1111/pim.12111 |
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Willey |
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Willey |
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