Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli
| Main Author: | |
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| Publication Date: | 2022 |
| Other Authors: | , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.4103/1995-7645.338437 |
| Download full: | https://arca.fiocruz.br/handle/icict/52577 |
Summary: | Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia,. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biodiversidade de Insetos e Patógenos. Niterói, RJ, Brasil. |
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Machado, Guilherme S.Lopes, Rosane L.Oliveira, Simone S. C.Freitas, Simone P. C.Santos-Mallet, Jacenir R.Santos, André L. S.Feder, DeniseGomes, Suzete A. O.2022-05-06T19:32:29Z2022-05-06T19:32:29Z2022MACHADO, Guilherme S. et al. Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli. Asian Pacific Journal of Tropical Medicine, v. 15, n. 2, p. 71- 78, May 2022.2352-4146https://arca.fiocruz.br/handle/icict/5257710.4103/1995-7645.338437engScienceDirect.com by ElsevierTrypanosoma rangeliRhodnius prolixusFolículo ovarianoVitelogeninaProtease aspárticaCisteína proteaseTrypanosoma rangeliRhodnius prolixusOvarian follicleVitellogeninAspartic proteaseCysteine proteaseUltrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeliinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia,. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biodiversidade de Insetos e Patógenos. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia,. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biodiversidade de Insetos e Patógenos. Niterói, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avanç ados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz Piauí. Teresina, PI, Brasil.Fundação Oswaldo Cruz Piauí. Teresina, PI, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica em Díptera e Hemíptera. Rio de Janeiro, RJ, Brasil / Universidade Iguaçu. Nova Iguaçu, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Geral. Laboratório de Estudos Avanç ados de Microrganismos Emergentes e Resistentes. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia,. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia,. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biodiversidade de Insetos e Patógenos. Niterói, RJ, Brasil.To investigate the morphological structure of ovarian follicular cells and biochemical parameters of both ovaries and fat bodies (sites of vitellogenesis) from Rhodnius (R.) prolixus infected with Trypanosoma (T.) rangeli. Methods: Adult virgin females of R. prolixus were fed upon a membrane apparatus containing heat-inactivated citrated rabbit blood and a suspension of T. rangeli epimastigotes (Macias strain). Females from the control group and all the males received parasitefree blood. Transmission electron microscopy was used to reveal the morphological aspects of ovarian follicle cells in both control and parasite-infected groups. Protein profile, proteolytic activities and Western blotting analyses were performed in either ovary or fat body samples of control and parasite-infected groups. Results: According to the ultrastructural data, T. rangeli infection elicited a degeneration process in the ovarian follicular cells of R. prolixus. Proteolytic assays indicated a reduction in the activity of aspartic peptidases in the ovary and fat body from parasite-infected group, while a significant increase in the cysteine peptidase activity was measured in both insect organs. Additionally, immunoblotting revealed that vitellogenin was overexpressed in the ovary of parasite-infected insects. Conclusions: T. rangeli infection seems to elicit an early programmed cell death in the ovarian follicle cells as well as induces the modulation on the activities of different peptidase classes in either ovaries or fat bodies and the overexpression of the vitellogenin in the ovary of R. prolixus.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/80b82805-eed0-499f-96ab-b11292861a36/download5a560609d32a3863062d77ff32785d58MD53falseAnonymousREADORIGINALJacenirsantos_mallet_etal_IOC_2022.pdfJacenirsantos_mallet_etal_IOC_2022.pdfapplication/pdf1246671https://arca.fiocruz.br/bitstreams/14b2424d-369f-4ff3-9ef8-0276f388fabc/download313f806ebd5c7d6a87902b9eae69475cMD54trueAnonymousREADTEXTJacenirsantos_mallet_etal_IOC_2022.pdf.txtJacenirsantos_mallet_etal_IOC_2022.pdf.txtExtracted texttext/plain41400https://arca.fiocruz.br/bitstreams/bc815814-4a2f-469e-8edc-f66f0020fc3e/download4c8ebb4267577777a36f6cff08944ee2MD59falseAnonymousREADTHUMBNAILJacenirsantos_mallet_etal_IOC_2022.pdf.jpgJacenirsantos_mallet_etal_IOC_2022.pdf.jpgGenerated Thumbnailimage/jpeg5373https://arca.fiocruz.br/bitstreams/fb6aade6-ec11-407c-bee5-8580586ebbba/download5fe4a510691f68e91f7ff8f04b74208fMD510falseAnonymousREADicict/525772025-07-29 18:55:28.13open.accessoai:arca.fiocruz.br:icict/52577https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-29T21:55:28Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.none.fl_str_mv |
Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli |
| title |
Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli |
| spellingShingle |
Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli Machado, Guilherme S. Trypanosoma rangeli Rhodnius prolixus Folículo ovariano Vitelogenina Protease aspártica Cisteína protease Trypanosoma rangeli Rhodnius prolixus Ovarian follicle Vitellogenin Aspartic protease Cysteine protease |
| title_short |
Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli |
| title_full |
Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli |
| title_fullStr |
Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli |
| title_full_unstemmed |
Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli |
| title_sort |
Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli |
| author |
Machado, Guilherme S. |
| author_facet |
Machado, Guilherme S. Lopes, Rosane L. Oliveira, Simone S. C. Freitas, Simone P. C. Santos-Mallet, Jacenir R. Santos, André L. S. Feder, Denise Gomes, Suzete A. O. |
| author_role |
author |
| author2 |
Lopes, Rosane L. Oliveira, Simone S. C. Freitas, Simone P. C. Santos-Mallet, Jacenir R. Santos, André L. S. Feder, Denise Gomes, Suzete A. O. |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Machado, Guilherme S. Lopes, Rosane L. Oliveira, Simone S. C. Freitas, Simone P. C. Santos-Mallet, Jacenir R. Santos, André L. S. Feder, Denise Gomes, Suzete A. O. |
| dc.subject.other.none.fl_str_mv |
Trypanosoma rangeli Rhodnius prolixus Folículo ovariano Vitelogenina Protease aspártica Cisteína protease |
| topic |
Trypanosoma rangeli Rhodnius prolixus Folículo ovariano Vitelogenina Protease aspártica Cisteína protease Trypanosoma rangeli Rhodnius prolixus Ovarian follicle Vitellogenin Aspartic protease Cysteine protease |
| dc.subject.en.none.fl_str_mv |
Trypanosoma rangeli Rhodnius prolixus Ovarian follicle Vitellogenin Aspartic protease Cysteine protease |
| description |
Universidade Federal Fluminense. Instituto de Biologia. Programa de Pós-Graduação em Ciências e Biotecnologia,. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biodiversidade de Insetos e Patógenos. Niterói, RJ, Brasil. |
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2022 |
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2022-05-06T19:32:29Z |
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2022-05-06T19:32:29Z |
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2022 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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MACHADO, Guilherme S. et al. Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli. Asian Pacific Journal of Tropical Medicine, v. 15, n. 2, p. 71- 78, May 2022. |
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https://arca.fiocruz.br/handle/icict/52577 |
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2352-4146 |
| dc.identifier.doi.none.fl_str_mv |
10.4103/1995-7645.338437 |
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MACHADO, Guilherme S. et al. Ultrastructural and enzymatic alterations in the ovary of Rhodnius prolixus infected with Trypanosoma rangeli. Asian Pacific Journal of Tropical Medicine, v. 15, n. 2, p. 71- 78, May 2022. 2352-4146 10.4103/1995-7645.338437 |
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https://arca.fiocruz.br/handle/icict/52577 |
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eng |
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ScienceDirect.com by Elsevier |
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ScienceDirect.com by Elsevier |
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