Decreased endothelial nitric oxide, systemic oxidative stress, and increased sympathetic modulation contribute to hypertension in obese rats
| Main Author: | |
|---|---|
| Publication Date: | 2014 |
| Other Authors: | , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1152/ajpheart.00520.2013 |
| Download full: | https://arca.fiocruz.br/handle/icict/16048 |
Summary: | Fundo de Auxílio ao Ensino, Pesquisa e Extensão da Universidade Estadual de Londrina, Brazil, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Fundação de Amparo a Pesquisa do Estado de São Paulo, and CNPq Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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Cunha, Natalia Veronez daPinge-Filho, PhilenoPanis, CarolinaSilva, Bruno RodriguesPernomian, LaenaGrando, Marcella DarugeCecchini, RubensBendhack, Lusiane MariaMartins-Pinge, Marli Cardoso2016-10-03T15:31:44Z2016-10-03T15:31:44Z2014CUNHA, Natalia Veronez da et al. Decreased endothelial nitric oxide, systemic oxidative stress, and increased sympathetic modulation contribute to hypertension in obese rats. Am J Physiol Heart Circ Physiol. n. 306, p. H1472-H1480, 2014.0363-6135https://arca.fiocruz.br/handle/icict/1604810.1152/ajpheart.00520.2013Fundo de Auxílio ao Ensino, Pesquisa e Extensão da Universidade Estadual de Londrina, Brazil, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Fundação de Amparo a Pesquisa do Estado de São Paulo, and CNPq Conselho Nacional de Desenvolvimento Científico e TecnológicoUniversidade Estadual de Londrina. Departamento de Ciências Fisiológicas. Londrina, PR, Brasil.Universidade Estadual de Londrina. Departamento de Ciências Patológicas. Londrina, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Departamento de Ciências Patológicas. Londrina, PR, Brasil.Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Escola de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto, SP, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Física e Química. Ribeirão Preto, SP, Brasil.Universidade Estadual de Londrina. Departamento de Ciências Patológicas. Londrina, PR, Brasil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Física e Química. Ribeirão Preto, SP, Brasil.Universidade Estadual de Londrina. Departamento de Ciências Fisiológicas. Londrina, PR, Brasil.We investigated the involvement of nitric oxide (NO) and reactive oxygen species (ROS) on autonomic cardiovascular parameters, vascular reactivity, and endothelial cells isolated from aorta of monosodium glutamate (MSG) obese rats. Obesity was induced by administration of 4 mg/g body wt of MSG or equimolar saline [control (CTR)] to newborn rats. At the 60th day, the treatment was started with N(G)-nitro-L-arginine methyl ester (L-NAME, 20 mg/kg) or 0.9% saline. At the 90th day, after artery catheterization, mean arterial pressure (MAP) and heart rate were recorded. Plasma was collected to assess lipid peroxidation. Endothelial cells isolated from aorta were evaluated by flow cytometry and fluorescence intensity (FI) emitted by NO-sensitive dye [4,5-diaminofluoresceindiacetate (DAF-2DA)] and by ROS-sensitive dye [dihydroethidium (DHE)]. Vascular reactivity was made by concentration-response curves of acetylcholine. MSG showed hypertension compared with CTR. Treatment with L-NAME increased MAP only in CTR. The MSG induced an increase in the low-frequency (LF) band and a decrease in the high-frequency band of pulse interval. L-NAME treatment increased the LF band of systolic arterial pressure only in CTR without changes in MSG. Lipid peroxidation levels were higher in MSG and were attenuated after L-NAME. In endothelial cells, basal FI to DAF was higher in CTR than in MSG. In both groups, acetylcholine increased FI for DAF from basal. The FI baseline to DHE was higher in MSG than in CTR. Acetylcholine increased FI to DHE in the CTR group, but decreased in MSG animals. We suggest that reduced NO production and increased production of ROS may contribute to hypertension in obese MSG animals.engIrving H. ZuckerDecreased endothelial nitric oxide, systemic oxidative stress, and increased sympathetic modulation contribute to hypertension in obese ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlenitric oxide synthaseendotheliumautonomichypertensionmonosodium glutamateÓxido Nítrico SintaseEndotélioGlutamato de SódioHipertensãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://arca.fiocruz.br/bitstreams/ccd1168b-b0a2-4ad8-afe1-8c6bea3bcac2/download0271fb68963c017aa933c7aff60379f4MD51falseAnonymousREADORIGINALDecreased endothelial nitric oxide.pdfDecreased endothelial nitric oxide.pdfapplication/pdf253893https://arca.fiocruz.br/bitstreams/f9a65183-f5b2-4ff2-8793-db917c035548/download48279c58ff340e5623f8fd3b0b140153MD52trueAnonymousREADTEXTDecreased endothelial nitric oxide.pdf.txtDecreased endothelial nitric oxide.pdf.txtExtracted texttext/plain51989https://arca.fiocruz.br/bitstreams/446c4b45-bdcb-4f96-87cd-18da2e8faab0/download3533bd0ebcc82836e3dbddcecbbf99a1MD55falseAnonymousREADTHUMBNAILDecreased endothelial nitric oxide.pdf.jpgDecreased endothelial nitric oxide.pdf.jpgGenerated Thumbnailimage/jpeg6692https://arca.fiocruz.br/bitstreams/0f2d6ff7-5f06-4635-acc8-4fb7298d953e/download7c13e735919e93db749983a9f58e110bMD56falseAnonymousREADicict/160482025-07-29 21:33:16.35open.accessoai:arca.fiocruz.br:icict/16048https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T00:33:16Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.none.fl_str_mv |
Decreased endothelial nitric oxide, systemic oxidative stress, and increased sympathetic modulation contribute to hypertension in obese rats |
| title |
Decreased endothelial nitric oxide, systemic oxidative stress, and increased sympathetic modulation contribute to hypertension in obese rats |
| spellingShingle |
Decreased endothelial nitric oxide, systemic oxidative stress, and increased sympathetic modulation contribute to hypertension in obese rats Cunha, Natalia Veronez da nitric oxide synthase endothelium autonomic hypertension monosodium glutamate Óxido Nítrico Sintase Endotélio Glutamato de Sódio Hipertensão |
| title_short |
Decreased endothelial nitric oxide, systemic oxidative stress, and increased sympathetic modulation contribute to hypertension in obese rats |
| title_full |
Decreased endothelial nitric oxide, systemic oxidative stress, and increased sympathetic modulation contribute to hypertension in obese rats |
| title_fullStr |
Decreased endothelial nitric oxide, systemic oxidative stress, and increased sympathetic modulation contribute to hypertension in obese rats |
| title_full_unstemmed |
Decreased endothelial nitric oxide, systemic oxidative stress, and increased sympathetic modulation contribute to hypertension in obese rats |
| title_sort |
Decreased endothelial nitric oxide, systemic oxidative stress, and increased sympathetic modulation contribute to hypertension in obese rats |
| author |
Cunha, Natalia Veronez da |
| author_facet |
Cunha, Natalia Veronez da Pinge-Filho, Phileno Panis, Carolina Silva, Bruno Rodrigues Pernomian, Laena Grando, Marcella Daruge Cecchini, Rubens Bendhack, Lusiane Maria Martins-Pinge, Marli Cardoso |
| author_role |
author |
| author2 |
Pinge-Filho, Phileno Panis, Carolina Silva, Bruno Rodrigues Pernomian, Laena Grando, Marcella Daruge Cecchini, Rubens Bendhack, Lusiane Maria Martins-Pinge, Marli Cardoso |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cunha, Natalia Veronez da Pinge-Filho, Phileno Panis, Carolina Silva, Bruno Rodrigues Pernomian, Laena Grando, Marcella Daruge Cecchini, Rubens Bendhack, Lusiane Maria Martins-Pinge, Marli Cardoso |
| dc.subject.en.none.fl_str_mv |
nitric oxide synthase endothelium autonomic hypertension monosodium glutamate |
| topic |
nitric oxide synthase endothelium autonomic hypertension monosodium glutamate Óxido Nítrico Sintase Endotélio Glutamato de Sódio Hipertensão |
| dc.subject.decs.none.fl_str_mv |
Óxido Nítrico Sintase Endotélio Glutamato de Sódio Hipertensão |
| description |
Fundo de Auxílio ao Ensino, Pesquisa e Extensão da Universidade Estadual de Londrina, Brazil, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Fundação de Amparo a Pesquisa do Estado de São Paulo, and CNPq Conselho Nacional de Desenvolvimento Científico e Tecnológico |
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2014 |
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2014 |
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2016-10-03T15:31:44Z |
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2016-10-03T15:31:44Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
CUNHA, Natalia Veronez da et al. Decreased endothelial nitric oxide, systemic oxidative stress, and increased sympathetic modulation contribute to hypertension in obese rats. Am J Physiol Heart Circ Physiol. n. 306, p. H1472-H1480, 2014. |
| dc.identifier.uri.fl_str_mv |
https://arca.fiocruz.br/handle/icict/16048 |
| dc.identifier.issn.none.fl_str_mv |
0363-6135 |
| dc.identifier.doi.none.fl_str_mv |
10.1152/ajpheart.00520.2013 |
| identifier_str_mv |
CUNHA, Natalia Veronez da et al. Decreased endothelial nitric oxide, systemic oxidative stress, and increased sympathetic modulation contribute to hypertension in obese rats. Am J Physiol Heart Circ Physiol. n. 306, p. H1472-H1480, 2014. 0363-6135 10.1152/ajpheart.00520.2013 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.publisher.none.fl_str_mv |
Irving H. Zucker |
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Irving H. Zucker |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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