Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis.
| Main Author: | |
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| Publication Date: | 2025 |
| Other Authors: | , , , , , , , , , |
| Format: | Article |
| Language: | eng |
| Source: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.3390/biomedicines13030587 |
| Download full: | https://arca.fiocruz.br/handle/icict/69630 |
Summary: | Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. |
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Moreira, Thayse BatistaSilvestrini, Marina Malheiros AraujoMagalhães, Ana Luiza de FreitasRangel, Kerstin KappCosta, Alvaro PercinioGomes, Matheus SouzaAmaral, Laurence Rodrigues doMartins Filho, Olindo AssisSalles, Paulo Guilherme de OliveiraBraga, Leticia ConceiçãoCarvalho, Andréa Teixeira de2025-04-14T17:23:17Z2025-04-14T17:23:17Z2025MOREIRA, Thayse Batista et al. Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis. Biomedicines, v. 13, n. 3, 587, 2025.2227-9059https://arca.fiocruz.br/handle/icict/6963010.3390/biomedicines13030587engMDPINeutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil.Instituto Mario Penna. Hospital Luxemburgo. Belo Horizonte, MG, Brasil.Instituto Mario Penna. Hospital Luxemburgo. Belo Horizonte, MG, Brasil.Instituto Mario Penna. Hospital Luxemburgo. Belo Horizonte, MG, Brasil.Universidade Federal de Uberlândia. Laboratório de Bioinformática e Analise Molecular. Patos de Minas, MG, Brasil.Universidade Federal de Uberlândia. Laboratório de Bioinformática e Analise Molecular. Patos de Minas, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil.Instituto Mario Penna. Hospital Luxemburgo. Belo Horizonte, MG, Brasil. / Instituto Mario Penna. Hospital Luxemburgo. Laboratório de Anatomia Patológica. Belo Horizonte, MG, Brasil.Instituto Mario Penna. Instituto de Ensino, Pesquisa e Inovação. Laboratório de Pesquisa Translacional em Oncologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil.Introduction: Breast cancer (BC) is a disease that affects about 2.2 million people worldwide. The prognosis and treatment of these patients depend on clinical and histopathologic staging, in which more aggressive cancers need a less conservative therapeutic approach. Previous studies showed that patients with BC have an increased frequency of systemic microvesicles (MVs) that are associated with invasion, progression, and metastasis, which can be used in liquid biopsy to predict the therapeutic response in individualized treatment. Objective: This study proposes the development of a minimally invasive BC diagnostic panel and follow-up biomarkers as a complementary method to screen patients. Methods: The quantification of circulating MVs in 48 healthy women and 100 BC patients who attended the Mario Penna Institute between 2019 and 2022 was performed by flow cytometry. In addition, the MVs of BC patients were analyzed before treatment and 6, 12, and 24 months post-treatment. Machine learning approaches were employed to determine the performance of MVs to identify BC and to propose BC classifier algorithms. Results: Patients with BC had more neutrophil- and endothelial cell-derived MVs than controls before treatment. After treatment, all MV populations were decreased compared to pre-treatment, but leukocyte- and erythrocyte-derived MVs were increased at 12 months after treatment, before decreasing again at 24 months. Conclusions: Performance analyses and machine learning approaches pointed out that MVs from neutrophils and endothelial cells are the best candidates for BC diagnostic biomarkers. Neutrophil- and endothelial cell-derived MVs are putative candidates for BC biomarkers to be employed as screening tests for BC diagnosis.Breast cancerLiquid biopsyExtracellular microvesiclesFlow cytometryBiomarkersinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://arca.fiocruz.br/bitstreams/463205c8-3626-426b-a1d5-59b621948088/download5a560609d32a3863062d77ff32785d58MD51falseAnonymousREADORIGINALNetrophil.pdfNetrophil.pdfapplication/pdf11560403https://arca.fiocruz.br/bitstreams/0717ba5a-631c-4294-a021-725a9447c11c/download8b7eda2e6f2b5a693ef3c93dc2813824MD52trueAnonymousREADTEXTNetrophil.pdf.txtNetrophil.pdf.txtExtracted texttext/plain44472https://arca.fiocruz.br/bitstreams/1e9b39f8-2a8f-48f9-ad99-d4ddccbd6c2c/download7feb8360e44a84b3cb226c6017d07355MD55falseAnonymousREADTHUMBNAILNetrophil.pdf.jpgNetrophil.pdf.jpgGenerated Thumbnailimage/jpeg5542https://arca.fiocruz.br/bitstreams/03bf9f7b-96c4-4b3f-b0f3-38362af5fd22/download6391889f1ee3c574952016a9354ed36dMD56falseAnonymousREADicict/696302025-07-29 23:00:24.395open.accessoai:arca.fiocruz.br:icict/69630https://arca.fiocruz.brRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352025-07-30T02:00:24Repositório Institucional da FIOCRUZ (ARCA) - 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| dc.title.none.fl_str_mv |
Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis. |
| title |
Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis. |
| spellingShingle |
Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis. Moreira, Thayse Batista Breast cancer Liquid biopsy Extracellular microvesicles Flow cytometry Biomarkers |
| title_short |
Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis. |
| title_full |
Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis. |
| title_fullStr |
Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis. |
| title_full_unstemmed |
Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis. |
| title_sort |
Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis. |
| author |
Moreira, Thayse Batista |
| author_facet |
Moreira, Thayse Batista Silvestrini, Marina Malheiros Araujo Magalhães, Ana Luiza de Freitas Rangel, Kerstin Kapp Costa, Alvaro Percinio Gomes, Matheus Souza Amaral, Laurence Rodrigues do Martins Filho, Olindo Assis Salles, Paulo Guilherme de Oliveira Braga, Leticia Conceição Carvalho, Andréa Teixeira de |
| author_role |
author |
| author2 |
Silvestrini, Marina Malheiros Araujo Magalhães, Ana Luiza de Freitas Rangel, Kerstin Kapp Costa, Alvaro Percinio Gomes, Matheus Souza Amaral, Laurence Rodrigues do Martins Filho, Olindo Assis Salles, Paulo Guilherme de Oliveira Braga, Leticia Conceição Carvalho, Andréa Teixeira de |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Moreira, Thayse Batista Silvestrini, Marina Malheiros Araujo Magalhães, Ana Luiza de Freitas Rangel, Kerstin Kapp Costa, Alvaro Percinio Gomes, Matheus Souza Amaral, Laurence Rodrigues do Martins Filho, Olindo Assis Salles, Paulo Guilherme de Oliveira Braga, Leticia Conceição Carvalho, Andréa Teixeira de |
| dc.subject.en.none.fl_str_mv |
Breast cancer Liquid biopsy Extracellular microvesicles Flow cytometry Biomarkers |
| topic |
Breast cancer Liquid biopsy Extracellular microvesicles Flow cytometry Biomarkers |
| description |
Fundação Oswaldo Cruz. Instituto Rene Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brasil. |
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2025 |
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2025-04-14T17:23:17Z |
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2025-04-14T17:23:17Z |
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2025 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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MOREIRA, Thayse Batista et al. Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis. Biomedicines, v. 13, n. 3, 587, 2025. |
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https://arca.fiocruz.br/handle/icict/69630 |
| dc.identifier.issn.none.fl_str_mv |
2227-9059 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/biomedicines13030587 |
| identifier_str_mv |
MOREIRA, Thayse Batista et al. Neutrophil-and Endothelial Cell-Derived Extracellular Microvesicles Are Promising Putative Biomarkers for Breast Cancer Diagnosis. Biomedicines, v. 13, n. 3, 587, 2025. 2227-9059 10.3390/biomedicines13030587 |
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MDPI |
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MDPI |
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