The effect of TiF4 varnish after pre-treatment with MMP inhibitors on dentin erosion with or without demineralized organic matrix

• Ano de defesa: 2019
• Autor(a) principal: Vertuan, Mariele
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Biblioteca Digitais de Teses e Dissertações da USP
• Palavras-chave em Português: Dentin erosion; Erosão dentinária; Fluoride varnish; Proanthocyanidin; Proantocianidina; Verniz fluoretado
• Link de acesso: http://www.teses.usp.br/teses/disponiveis/25/25148/tde-27112019-163745/

This study tested the protective effect of TiF4 varnish, after pre-treatment with proanthocyanidin (PA) on the progression of erosive dentin wear, under the presence or absence of the demineralized organic matrix (DOM). For that, 360 bovine root dentin samples were polished and subjected to the analysis of the baseline profile. The samples were then eroded for 30 minutes (0.1% citric acid, pH 2.5) and the profile was measured again (erosion profile). Half of the samples were subjected to the DOM removal using collagenase for 4 days, while the other half remained immersed in water (with DOM). The removal of DOM was checked by profilometry (DOM profile). Samples were randomly divided into 24 groups (n= 15) according to the factors: 1 With or without DOM; 2 Pre-treatment with 0.012% chlorhexidine gel, 10% proanthocyanidin gel or untreated (control) for 1min; 3 Final Treatment with TiF4 varnish, NaF varnish, placebo varnish or untreated (control) for 6h. The samples were submitted to a pH cycling for 5 days: 0.1% citric acid (4x90s/day) and immersion in artificial saliva between the challenges. The final profile was obtained for the calculation of dentin wear (m, three-way ANOVA/Tukey test). When DOM was preserved, the erosive wear was lower (7.08m) compared to the condition without DOM (9.80m), regardless of the treatments (p=0.00). The pre-treatment had no influence on the amount of dentin wear (p=0.63), while the final treatment was effective in reducing the progression of dentin wear (about 18% compared to control, p=0.00). TiF4 was the only agent capable of reducing erosive dentin wear progression.