Detalhes bibliográficos
| Ano de defesa: |
2017 |
| Autor(a) principal: |
Sanches, Mariana Liessa Rovis
 |
| Orientador(a): |
Buzalaf, Camila Peres
|
| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade do Sagrado Coração
|
| Programa de Pós-Graduação: |
Ciência e Tecnologia Ambiental
|
| Departamento: |
Ciências Exatas e Sociais Aplicadas
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://tede2.usc.br:8080/jspui/handle/tede/342
|
Resumo: |
Methylmercury (MeHg) and dioxins, persistent toxic pollutants in the environment, are extensively associated with a number of health hazards. Several studies demonstrate an association between exposure to these elements and the development of the metabolic syndrome, a set of obesity-related diseases. These conditions are associated with altered metabolism of adipocytes, differentiated cells from mesenchymal stem cells and pre-adipocytes. However, little is known about the impact of these and its toxicants on adipogenic differentiation from 3T3-L1 pre-adipocytes. Thus, the objective of this study was to qualitatively and quantitatively evaluate the effect of in vitro exposure of MeHg and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) before and concurrent with adipogenic differentiation by deposition of Triglycerides, evaluated by fluorescence spectrometry and flow cytometry, in addition to the molecular mechanisms involved, by proteomic analysis. By both techniques, preadipocyte exposure to MeHg and TCDD in non-cytotoxic doses, either alone or in combination, prior to the induction of adipogenic differentiation, did not significantly alter their ability to differentiate into mature adipocytes. However, when exposure to TCDD or combined with MeHg occurred concurrently with induction of differentiation, there was a significant reduction in the formation of mature cells. In addition, flow cytometry showed that the combination of both toxicants during induction of in vitro differentiation significantly reduced adipogenic differentiation with respect to each contaminant individually. Proteomic analysis showed that the profile and protein abundance relationships were altered according to the type of contaminant and the exposure phase, demonstrating that the contaminants act by different mechanisms and in a manner dependent on the period of exposure |
