Biomonitoramento citogenético em pacientes periodontais portadores de Diabetes Mellitus tipo 2

• Ano de defesa: 2012
• Autor(a) principal: Cavezzi Junior, Orlando
• Orientador(a): Saraiva, Patricia Pinto
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: IASCJ - Universidade Sagrado Coração
• Programa de Pós-Graduação: Biologia Oral
• Departamento: Ciências da Saúde e Biológicas
• País: BR
• Palavras-chave em Português: DOENÇA PERIODONTAL; DIABETES MELLITUS; ALTERAÇÃO NUCLEAR; MICRONÚCLEO
• Palavras-chave em Inglês: PERIODONTAL DISEASE; DIABETES MELLITUS; NUCLEAR DAMAGE; MICRONUCLEUS
• Área do conhecimento CNPq: CIENCIAS DA SAUDE::ODONTOLOGIA
• Link de acesso: http://localhost:8080/tede/handle/tede/138

Both periodontal disease such as diabetes produce chemical mediators that expose the oral tissues to stress, causing damage to genetic material in epitelial cells of the oral mucosa, which are early signs of developing oral cancer. Our objective was to evaluate DNA damage and / or cell death promoted by frequente exposure to chemical inflammatory mediators and oxidative stress, due to the association between periodontal disease and diabetes mellitus, using the micronucleus test. This investigation included 60 patients divided into three groups. Group 1 (control) composed of 20 patients who had absence of periodontitis and diabetes mellitus. Group 2 composed of 20 patients with periodontitis and absence of diabetes mellitus (fasting glucose 70 to 99 mg / dL and glycated hemoglobin (HbA1c) <6%) and group 3 composed of 20 patients with periodontitis and diabetes mellitus type 2 , with fasting glucose greater than 126mg/dl and glycosylated hemoglobina (HbA1c)> 6%. Our results showed that the association between periodontal disease and diabetes promotes an increase in the occurrence of micronuclei (5.4 ± 0.99, p <0.05) compared to patients with periodontal disease alone (4 ± 0.85) patients and healthy (no periodontal disease and without diabetes) (1.85 ± 0.74). The other nuclear alterations observed, indicative of cell death, were also present in greater frequency in the association of periodontal disease and diabetes (20.85 ± 2, p <0.001), compared to only patients with periodontal disease (14.45 ± 1 , 82), and healthy patients (6.55 ± 1). A moderately positive correlation (P <0.001) between the plaque index, gingival index and the occurrence of nuclear changes analyzed. These data suggest a direct association between the chemical mediators produced periodontal disease on the oral mucosa cells, this damage is intensified when diabetes occurs simultaneously. These results indicate that patients with periodontal disease and diabetes mellitus were more likely to have nuclear damage, leaving these patients more vulnerable to the development of oral cancer.