Detalhes bibliográficos
| Ano de defesa: |
2007 |
| Autor(a) principal: |
Pazzini, Dalva
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| Orientador(a): |
Zurron, Ana Claudia Bensuaski de Paula
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| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
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| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
IASCJ - Universidade Sagrado Coração
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| Programa de Pós-Graduação: |
Biologia Oral
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| Departamento: |
Ciências da Saúde e Biológicas
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| País: |
BR
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| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://localhost:8080/tede/handle/tede/154
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Resumo: |
Maytenus ilicifolia, (Celastraceae) known as espinheira-santa , is very used in the popular medicine for peptic ulcer treatment. Several studies were made to evaluate the anti- inflammatory, antiulcerogenic and antifungus activity with absence of toxic effects. This work evaluates the preventive and healing actions of M. ilicifolia in animals, as well as the antioxidative activity by Electron Spin Resonance spectroscopy (RSE). In our study, Maytenus ilicifolia (300 and 500 mg/Kg-1, p.o.) prevented the gastric lesions formation in 42,2% and 72,0% (p <0,05 and p <0,001, respectively) when compared with the negative control tween 80. Therefore, the oral administration of M. ilicifolia one hour before the ulcerogenic agent prevented the ulcer formation, conserving the citoprotection characteristics of the gastric mucosa and assuring the integrity of gastric glands and gastric fossets. In this same experiment, the animals treated with the negative control tween, had the epithelium and the mucosa layer damaged. It was also observed exfoliation of the surface epithelium and accentuated vascularization. The healing activity of M.ilicifolia (500mg/kg-1, p.o.) was 63% (p <0,001) in chronic ulcer experiments induced by the acetic acid. The histological analysis confirmed the recovery of the mucosal layer (gastric glands and gastric fossets) and the surface epithelium harmed by the acetic acid. In the study of chronic ulcer induction by ethanol (25%), it was possible to verify that the mother's gastric mucosal presented severe lesions and intense vascularization. It was verified an accentuated degeneration of the brush border epithelium, reducing the intestinal absorption of these animals. The pups presented neither gastric lesions nor intestinal epithelium degeneration, showing that they have adapted to the ulcerogenic process inside of the maternal uterus. Studies in vitro, conducted with an Electron Spin Resonance spectroscopy, proved the antioxidative action of M ilicifolia. Experiments with DPPH (2,2-Diphenyl -1- picrylhydrazyl) demonstrate that the polar fraction of M. ilicifolia (1,0 mg/ml) presents a inhibitory effect of 50,78%. The experiments with the radical OH, produced by the Fenton Reaction and detected through DMPO-OH, showed that M. ilicifolia (5mg/ml) presents a inhibitory effect of 46,35%. The maximum inhibitory effect of 90,47% was obtained with 50 mg/ml of the polar fraction of M. ilicifolia. |
