Detalhes bibliográficos
| Ano de defesa: |
2017 |
| Autor(a) principal: |
FALCÃO, Marcus Vinícius Dias
 |
| Orientador(a): |
MOTA, Rinaldo Aparecido |
| Banca de defesa: |
MOTA, Rinaldo Aparecido,
SANTANA, Vania Lucia de Assis,
MAIA, Rita de Cassia Carvalho |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal Rural de Pernambuco
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Biociência Animal
|
| Departamento: |
Departamento de Morfologia e Fisiologia Animal
|
| País: |
Brasil
|
| Palavras-chave em Português: |
|
| Área do conhecimento CNPq: |
|
| Link de acesso: |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/7215
|
Resumo: |
Brucellosis is an infectious disease with a chronic characteristic caused by the bacteria Brucella spp. The aim of this study was to develop and standardize a Western Blotting technique for use in bovine brucellosis serodiagnosis. Two groups were used: group I with 60 samples of true positive and true negative animals being 30 positive and 30 negative in CFT and group II with 383 field samples being 90 positive and 293 negative in CFT. The analysis using WB technique revealed the molecular weight ≤20 kDa as the most characteristic and reactive area for identification and separation of animals with natural infection from vaccinated. The sensitivity and specificity of WB as compared to AAT were 93% and 99% respectively, and when compared to TFC respectively were 97% and 98%. The WB standardized in this study may be used as confirmatory test, because it is more sensitive and specific with capacity for differentiation between naturally infected and vaccinated animals. |
