Caracterização biológica, morfológica e molecular de isolados de Colletotrichum spp. associados à antracnose da cebola

Detalhes bibliográficos
Ano de defesa: 2015
Autor(a) principal: LOPES, Luiz Henrique Rocha lattes
Orientador(a): REIS, Ailton
Banca de defesa: NOVA, Meiriana Xavier Vila, MARIANO, Rosa de Lima Ramos, GAMA, Marcus Aurélio Siqueira da
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural de Pernambuco
Programa de Pós-Graduação: Programa de Pós-Graduação em Fitopatologia
Departamento: Departamento de Agronomia
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/6618
Resumo: One of the main onion crop diseases in Brazil is anthracnose, caused by especies of genus Colletotrichum. In this work it was used several Colletotrichum isolates obtained from plants do Allium genus with symptoms of anthracnose. The isolates were obtained from different regions of Brazil and characterized with the aid of morphological methods, molecular and cultural features. The Bayesian Analysis Method of inference was used for an initial analysis involving the genomic region β-tubulin (tub2). A representative group of isolates, selected in the first analysis, was sequenced for the partial region of actin (act) and ApMat. Two groups of haplotypes were generated and analyzed separately, one by concatenating tub2 and act and Other tub2, act and ApMat. The analysis revealed along with morphological analyzes that onion anthracnose is probably caused by several Colletotrichum species belonging to complexes acutatum and gloesporioides. All the representative isolates used in this study were pathogenic on onion, being able to cause of induce typical symptoms of anthracnose, especially on bulbs with variable aggressiveness range. All the results obtained in this work lead us to believe that onion anthracnose is caused by several species different of what has been reported so far.