Detalhes bibliográficos
| Ano de defesa: |
2014 |
| Autor(a) principal: |
SILVA, Vanessa Carla Lima da
 |
| Orientador(a): |
LIMA, Evilda Rodrigues de |
| Banca de defesa: |
GOMES, Lucas Alécio,
MOTA, Rinaldo Aparecido,
ALVES, Leucio Câmara |
| Tipo de documento: |
Tese
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal Rural de Pernambuco
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciência Veterinária
|
| Departamento: |
Departamento de Medicina Veterinária
|
| País: |
Brasil
|
| Palavras-chave em Português: |
|
| Área do conhecimento CNPq: |
|
| Link de acesso: |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/8621
|
Resumo: |
The present objective was to characterize the clinical, epidemiological, and hematological changes, and the molecular diagnosis using conventional PCR and RT-PCR in dogs naturally infected with Ehrlichia canis, Babesia canis vogeli and by CDV. One hundred and forty-six dogs were selected, regardless of gender and of varying ages, that had at least three clinical signs suggestive of these infections. Blood was collected for hemogram, blood parasitology, investigation of viral inclusion, PCR for detection of E. canis, B. canis vogeli and RT-PCR for CDV. The results obtained regarding infections by Ehrlichia canis, Babesia canis vogeli and CDV were 17.1%, 4.8%, and 27.4%, respectively. Co-infections by E. canis and B. canis vogeli were observed in 0.7%, Ehrlichia canis and CDV in 7.5%, and 2.1% of the animals were co-infected by all three infectious agents.Females presented a higher percentage of positive results for ehrlichiosis (26.4%) and distemper (34.7%), while males had a higher percentage for babesiosis with 8.1%. Animals up to 12 months and with specific breeds had the highest percentage for ehrlichiosis and distemper, while those above 84 months had more positives for babesiosis. Dogs examined at the Veterinary Hospital with ehrlichiosis, babesiosis and canine distemper, domiciled, vaccinated breed were most affected and street access may be liable as an important risk factor for infection in dogs with CDV. The clinical changes hyporexia, periocular alopecia, lymphadenopathy and unproductive cough should be considered in the characterization of ehrlichiosis; pale mucous for babesiosis; lymphadenopathy, congested mucous, ataxia and nasal discharge for distemper. Anemia, thrombocytopenia, eosinophilia, and lymphocytopenia should be considered as a source of variation in the characterization of ehrlichiosis; thrombocytopenia and neutropenia for babesiosis and eosinophilia for distemper. In co-infection for Ehrlichia canis with the CDV is destaracam ophthalmic disorders, gastrointestinal, anemia, thrombocytopenia, leukopenia, eosinopenia and lymphocytopenia. The number of animals co-infected by Ehrlichia canis and Babesia canis vogeli; E. canis, B. canis vogeli and CDV were insufficient to characterize the findings clinical and laboratory, suggesting that further studies be made in this direction. There was poor agreement between the PCR compared to blood parasite examination and no agreement for RT-PCR and viral inclusion research, should the clinician requesting the PCR, to be more specific and sensitive. Therefore, attention must be given to the possibility of co-infections, especially in endemic areas, and further studies are suggested so that other possibilities of simultaneous infections in small animal medicine can be clarified since this subject is not sufficiently studied in Brazil. |
