Detalhes bibliográficos
| Ano de defesa: |
2007 |
| Autor(a) principal: |
XAVIER, Guadalupe de Carvalho |
| Orientador(a): |
GUERRA, Maria Madalena Pessoa |
| Banca de defesa: |
BICUDO, Sony Dimas,
NEVES, Elizabeth da Silveira,
VIEIRA, Rômulo José |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal Rural de Pernambuco
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciência Veterinária
|
| Departamento: |
Departamento de Medicina Veterinária
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5674
|
Resumo: |
Aiming to evaluate the effect of diet supplementation with Selenium and Vitamin E (Selevit E®) on the testis and seminal parameters of goats induced to scrotal insulation, it were used 12 animals with age varying between seven and eight months, distributed randomly in two groups (G1= Control; G2= Selenium and Vitamin E) and submitted to adaptation period of 60 days. In this period the animals were submitted to two semen harvests and weighted each seven days to supplement and diet correction on the G2 animals. Before putting the testis plastic bags to SI, it was measured scrotal circumference of all animals. Animals of both groups were induced to SI during 18 days, period of which they were submitted to six semen harvests, one before SI (0d), one at the finish of SI (18d) and four on the post-insulation period (PSI). At the end of SI, it was chosen randomly three animals of each group to be submitted to bilateral orchiectomy. The G2 animals have diet supplementation with Selenium and Vitamin E during this period. The maintenance diet was offered to both groups during 42 days, corresponding to post-scrotal insulation (PSI). At the end of this period, the animals of G1 and G2 groups also were orchietomized to have biometric and volumetric testis parameters, where left and right testis as well as left and right epididymis were weighted and prepared to biometric and histometric analyses. The semen harvests were done by eletroejaculation method. The Seleniun and Vitamin E group animals have significantly high scrotal circumference at the end of SI, whereas the Selenium and Vitamin E group had values significantly higher of scrotal circumference at the end of SI, whereas the Control animals were more sensitive to degenerative effects of the SI on the same period. The SI time had significant effect on the quanti-qualitative characteristics of the semen on the animals of both groups (G1 and G2), with reduction of the evaluated parameters (PM, vigor, sperm concentration, acrosoma and DNA integrity), except to semen volume. At the same way it was observed damages like testis degeneration on orchiectomized animals of both groups. However, there was significant difference on the biometric and volumetric parameters of testis parenchyma only to blood vessel volume at this period. In contrast, the serum testosterone concentration increased highly at the end of SI and differed between groups (P<0.05). During the PSI period (36d PSI) there was no treatment effect on the seminal parameters, but it was observed day effect. However, at the end of PSI period the seminal parameters have values close to basal day (0d). The biometric and volumetric parameters of testis parenchyma, at the end of PSI period, have significant difference on the seminiferous tubules and seminiferous epithelium, as well as on the tubular diameter and ephitelium height of goats induced to scrotal insulation. It was observed also significant variation between groups to serum testosterone concentration. It can be concluded that the supplementation with Selenium and Vitamin E did not prevent drastic alterations caused by scrotal insulation technique. However, the continuous supplementation, 36 days after the end of SI, accelerated the spermatogenic recuperation process on goats. |
