Detalhes bibliográficos
Ano de defesa: |
2013 |
Autor(a) principal: |
SILVA, Mitaliene de Deus Soares da |
Orientador(a): |
HERCULANO, Polyanna Nunes |
Banca de defesa: |
RUFINO, Raquel Diniz,
GOMES, Bruno Severo |
Tipo de documento: |
Dissertação
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Tipo de acesso: |
Acesso aberto |
Idioma: |
por |
Instituição de defesa: |
Universidade Federal Rural de Pernambuco
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Programa de Pós-Graduação: |
Programa de Pós-Graduação em Biociência Animal
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Departamento: |
Departamento de Morfologia e Fisiologia Animal
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País: |
Brasil
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Palavras-chave em Português: |
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Palavras-chave em Inglês: |
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Área do conhecimento CNPq: |
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Link de acesso: |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/4685
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Resumo: |
Biosurfactant production was the selection factor between 20 yeasts isolated from bee pollen of Melipona seminigra merrilae of the lower Amazonas - AM. The production of biosurfactant by yeast 9II using waste as a source of carbon and nitrogen was optimized using statistical design Plackett-Burman. The metabolic liquid cell-free presented surface tension 28,30 mN/m, yield of 5,09 g/L of crude biosurfactant. The growth kinetics and production of the biosurfactant with the best production condition studied demonstrated a correlation between the growth of yeast with the biosurfactant production. The liquid metabolic presented emulsification of 100% for virgin engine oils and residual oil engine. The biosurfactant was stable before a pH range (2-12), temperature (4-120 °C) and NaCl concentrations (2-12%, w/v) and no toxicity compared to Lactuca sativa L., Brassica oleracea L. and Coriandrum sativum L. seeds and Artemia salina shrimp. |