Detalhes bibliográficos
| Ano de defesa: |
2007 |
| Autor(a) principal: |
SILVA, Flávio de Oliveira
 |
| Orientador(a): |
PORTO, Ana Lúcia Figueiredo |
| Banca de defesa: |
CUNHA, Maria das Graças Carneiro da,
BAPTISTA, Gandhi Rádis,
MOREIRA, Keila Aparecida |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Federal Rural de Pernambuco
|
| Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciência Veterinária
|
| Departamento: |
Departamento de Medicina Veterinária
|
| País: |
Brasil
|
| Palavras-chave em Português: |
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| Palavras-chave em Inglês: |
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| Área do conhecimento CNPq: |
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| Link de acesso: |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5664
|
Resumo: |
Lectins, carbohydrate binding proteins of non immune origin, agglutinate cells and glycoconjugates. Some proteins as the lectins from the Diocleinae tribe have described as pharmacological active molecules. Lectins form an important class of carbohydrate-binding proteins. Canavalia brasiliensis (ConBr) is a sugar-binding specificity [D-mannose (Dglucose)] protein. The aim of this study was evaluate the proteins profile and to evaluate histhopathologically treated wounds with ConBr seeds lectins. Surgical wounds were produced in the dorsal region in albino Swiss mice. Each wound was daily treated topically along 11 days, as follow: ConBr (10μg), ConBr/Manose (10μg in manose 0,1M), manose (0,1M) and NaCl (150mM). Protein profile has done through SDS-PAGE and showed proteins with molecular weight between 30 and 70KDa, suggesting the presence of metalloproteinases in the homogenates gotten from the cutaneous injuries. In 2nd day of postoperative (PO), the ConBr/Manose group was what it presented the highest proteolitic activity, in 7th day of P.O., the highest activity was observed in the ConBr and NaCl groups. Finally, in 12th day of PO ConBr/manose group showed a maxim proteolitic activity. ConBr group showed flogistic signs less intense than others groups, had presented contraction percentile superior during the fibroplasia phase. In the first 48 hours it was observed, in all the studied groups, the presence of polymorphonuclear leukocyte infiltrate, crust and angiogenesis. At 7th day there was presence of fibrovascular (manose, ConBr Manose and ConBr) and vascular granulation tissue (NaCl) in studied groups. Finally, at 12th day, wounds showed reepithelization. In ConBr/manose group there was presence of fibrovascular granulation tissue with more organized collagen network. Results suggests that wound healing it was better in ConBr group, having presented one better effect when used in association with manose. |
