Detalhes bibliográficos
Ano de defesa: |
2012 |
Autor(a) principal: |
AMARAL, Rodolfo Luiz Godoy do
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Orientador(a): |
SILVA, Leonildo Bento Galiza da |
Banca de defesa: |
PORTO, Wagnner José Nascimento,
Pinheiro Junior, José Wilton,
MOTA, Rinaldo Aparecido |
Tipo de documento: |
Dissertação
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Tipo de acesso: |
Acesso aberto |
Idioma: |
por |
Instituição de defesa: |
Universidade Federal Rural de Pernambuco
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Programa de Pós-Graduação: |
Programa de Pós-Graduação em Ciência Veterinária
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Departamento: |
Departamento de Medicina Veterinária
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País: |
Brasil
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Palavras-chave em Português: |
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Palavras-chave em Inglês: |
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Área do conhecimento CNPq: |
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Link de acesso: |
http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5852
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Resumo: |
The bovine neosporosis is an infectious disease caused by Neosporacaninum, obligate intracellular parasite, and is considered a major cause of bovine abortion in various countries. The objective was to study the occurrence of N. caninum in cows and fetuses in the states of Pernambuco and Alagoas, Brazil. We collected 306 blood serum samples from slaughtered cows and 30 fetuses in the states of Pernambuco and Alagoas. For serological diagnosis, we used the technique of immunofluorescence reaction (RIFI) with a cutoff 1:200 for sera of cows and fetal sera used cutoff 1:25 parasitic DNA research, we used tissue fetal submitted to the technique of Polymerase Chain Reaction (PCR). Serological assays, we observed 39/306 (12.6%) of the positive cows and 5/30 (16.7%) of positive fetuses. To detect the parasite 8/30 (26.6%) of fetuses were PCR positive. The results of this study as the presence of parasites in fetuses are unprecedented for this region and allow us to conclude that this agent should be included in the study of causes of bovine abortion in this region of Brazil. |