Pesquisa de Neospora caninum em vacas e fetos em matadouros nos estados de Pernambuco e Alagoas, Brasil

Detalhes bibliográficos
Ano de defesa: 2012
Autor(a) principal: AMARAL, Rodolfo Luiz Godoy do lattes
Orientador(a): SILVA, Leonildo Bento Galiza da
Banca de defesa: PORTO, Wagnner José Nascimento, Pinheiro Junior, José Wilton, MOTA, Rinaldo Aparecido
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural de Pernambuco
Programa de Pós-Graduação: Programa de Pós-Graduação em Ciência Veterinária
Departamento: Departamento de Medicina Veterinária
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Área do conhecimento CNPq:
Link de acesso: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5852
Resumo: The bovine neosporosis is an infectious disease caused by Neosporacaninum, obligate intracellular parasite, and is considered a major cause of bovine abortion in various countries. The objective was to study the occurrence of N. caninum in cows and fetuses in the states of Pernambuco and Alagoas, Brazil. We collected 306 blood serum samples from slaughtered cows and 30 fetuses in the states of Pernambuco and Alagoas. For serological diagnosis, we used the technique of immunofluorescence reaction (RIFI) with a cutoff 1:200 for sera of cows and fetal sera used cutoff 1:25 parasitic DNA research, we used tissue fetal submitted to the technique of Polymerase Chain Reaction (PCR). Serological assays, we observed 39/306 (12.6%) of the positive cows and 5/30 (16.7%) of positive fetuses. To detect the parasite 8/30 (26.6%) of fetuses were PCR positive. The results of this study as the presence of parasites in fetuses are unprecedented for this region and allow us to conclude that this agent should be included in the study of causes of bovine abortion in this region of Brazil.