Identificação de sexo em caprinos e ovinos através da técnica de PCR

Detalhes bibliográficos
Ano de defesa: 2008
Autor(a) principal: SANTOS JUNIOR, Edivaldo Rosas dos lattes
Orientador(a): WISCHRAL, Aurea
Banca de defesa: SILVA, Márcia Bezerra da, PORTO, Ana Lúcia Figueiredo, GOMES FILHO, Manoel Adrião
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Federal Rural de Pernambuco
Programa de Pós-Graduação: Programa de Pós-Graduação em Ciência Veterinária
Departamento: Departamento de Medicina Veterinária
País: Brasil
Palavras-chave em Português:
Palavras-chave em Inglês:
Sex
Área do conhecimento CNPq:
Link de acesso: http://www.tede2.ufrpe.br:8080/tede2/handle/tede2/5354
Resumo: The use of molecular biology in animal reproduction to identifying the sex genetic allows the producer has better reproductive planning, facilitating the coordination of actions aimed at rationalizing production and profitability. The objective of this study was to determine methods for the identification of the sex of the goats and sheep, using different concentrations of DNA (150, 100, 50, 1, 0.5, 0.2 and 0.1 ng) extracted from blood of both sexes, as a possibility for application in sexing embryos pre-implantating, sperm and animals pseudo-hermaphrodites. It was performed polymerase chain reaction (PCR) multiplex for the SRY gene (116bp) found in the Y chromosome, the gene for Aml-X (300pb) found on the X chromosome, in addition the sequences corresponding to genes ZFX / ZFY containing 445 and 447bp respectively. The use of these methods presented showed accuracy of 100% in samples of blood goat and sheep, in low concentrations, at least 0.2ng (SRY/Aml-X) and 1.0ng (SRY/ZFXZFY), which suggests the feasibility of the technique for determining sexual in these species.