Parâmetros clínicos periodontais e expressão genética em individuos com Diabetes Mellitus, dislipidemia e doença periodontal

• Ano de defesa: 2014
• Autor(a) principal: Oliveira, Rafael Nepomuceno [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Periodontia; Diabetes mellitus; Hiperlipidemia; Interleucina-10; Interferon; Genética; Periodontics
• Link de acesso: http://hdl.handle.net/11449/145505; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/21-09-2016/000806523.pdf

Diabetes mellitus type 2 and dyslipidemia are systemic diseases are involved in the pathogenesis of periodontal disease and may alter the immune-inflammatory response of the host. The aim of this study was to investigate the influence of DP, DM2 (compensated and decompensated) and dyslipidemia in systemic gene expression of immune- inflammatory response; and the correlation of these genes with periodontal clinical, glycemic and lipid profiles. Five groups of patients (with 30 individuals each) were investigated: DMdDisDP (decompensated diabetes, dyslipidemia and periodontal disease), DMcDisDP (compensated diabetes, dyslipidemia and periodontal disease), DisDP (only dyslipidemia and periodontal disease), cDP (periodontal disease only) and Control (without any of the three diseases). All patients underwent periodontal examination, physical examination and biochemical evaluation of lipid and glycemic profiles. From each patient, blood was collected and the RNA extracted. The cDNA was made to investigate the expression of genes involved in signaling pathways of IL-10, IFN-α and IFN-γ by Real Time PCR (qPCR). In patients with dyslipidemia, the expression of antiinflammatory genes was lower, meanwhile there was greater expression of proinflammatory genes. While anti-inflammatory genes correlated negatively with parameters of glucose and lipid profile, pro-inflammatory genes were positively correlated with these parameters. No differences were observed between patients with compensated and decompensated DM2. The DP did not appear to influence systemic expression of the investigated genes. We concluded that the dyslipidemia was the primary disease responsible for the difference in gene expression between groups.