Estresse e imuno modulação por beta-glucano em Pacu (Piaractus mesopotamicus)

Detalhes bibliográficos
Ano de defesa: 2014
Autor(a) principal: Sabioni, Rafael Estevan [UNESP]
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual Paulista (Unesp)
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://hdl.handle.net/11449/108914
Resumo: Fish farming intensification may increase the frequency and intensity of stressful situations, decreasing productivity. The stress response is established in order to maintain homeostasis, but its extension may decrease the ability of defense, by the action of cortisol, exposing fishes to pathogens. In this sense is studied the strategic use of molecules that have the ability to stimulate the immune system, such as ?-glucan, protecting the fish before stressful events. The immunity of fish is similar to that of mammals and can be monitored through the analysis of some components such as lysozyme, complement system proteins and white cells profile. Pacu was chosen as biological model by its productive characteristics. In this context three experiments were established. The first evaluated the action of ?-glucan-containing feed against a stress condition simulated by intraperitoneal injection of hydrocortisone. The second and third ones tested the action of the?-glucan against the elevation of exogenous and endogenous cortisol, respectively, and Aeromonas hydrophila inoculation. In the overall results, the protocols used to increase endogenous and exogenous cortisol were efficient, however, due to induce cortisol levels above the physiological, it is recommended to use a dose lower than 10mg / g hydrocortisone for the exogenous. The use of 0.5% ?-glucan reduced cortisol levels, suggesting attenuation of hormonal response, and activate leukocyte function with 0.1% and 0.5%, administered for 15 and 10 days, respectively, even with reduction of leukocytes number by cortisol action. The concentration of lysozyme and complement system activity responded to infection. increasing 24 hours after inoculation simultaneously