Genotipagem dos isolados de Giardia duodenalis em famílias de pescadores da colônia de Porto Said, Botucatu, São Paulo

• Ano de defesa: 2015
• Autor(a) principal: Arbex, Ana Paula Oliveira [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Giardia; Crianças - Doenças - Diagnostico; Diagnóstico molecular
• Link de acesso: http://hdl.handle.net/11449/132021; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/06-11-2015/000848444.pdf

The intestinal parasites persist as a public health problem, especially in developing countries, where the protozoan Giardia duodenalis (syn. Giardia intestinalis, Giardia lamblia) stands out as one of the most common causes of diarrhea in children. The present study was conducted to investigate the prevalence of Giardia and other intestinal parasites in children and workers of a daycare center of Vitoriana, a district of Botucatu municipality, São Paulo State, including also the household members and dogs of the children tested positive for Giardia. In addition, we proposed to investigate the genetic diversity of G. duodenalis infection in these population using three gene loci beta-giardin (bg), triose phosphate isomerase (tpi) and glutamate dehydrogenase (gdh). For this, three samples were analyzed from 123 children feces (0-6 years), 14 workers, 44 families members and 20 dogs. Samples were processed by centrifugal sedimentation and flotation and examined under a light microscope. Intestinal parasites were identified in 50 of 123 fecal samples from children (41.6%), and Giardia was the most frequent parasite detecte (21.9%). Out of the 27 families of children tested positive for Giardia, only 16 (44 individuals) provided stool samples. In these samples, cysts were detected in two samples. Focusing on Giardia infection among children attending day care, the analysis showed that younger children are at higher risk of acquiring Giardia infection (OR = 0.69; 95% CI = 00.49-0.97; p = 0.03). In addition, children living in families with a higher household density were more likely to be infected (OR = 1.8; 95% IC = 1.07-3.07; p = 0.03). DNA extracted from 186 stool samples (35 negative and 151 positive samples for microscopic examination) were amplified and the products sequenced generating 59 sequences as follow: 15 for bg, 25 for tpi and 19 for gdh. Out of the 29 isolates assessed, the sequencing analysis ...