Avaliação de polimorfismos genéticos na progressão da infecção de pacientes monoinfectados e coinfectados com os Vírus da Imunodeficiência Humana (HIV) e Vírus da Hepatite C (VHC)

• Ano de defesa: 2015
• Autor(a) principal: Massolini, Viviam Milanez [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: HIV (Vírus); Infecções por HIV; Hepatite por vírus; Coinfecção; Genes; HIV (Viruses); Hepatitis, Viral
• Link de acesso: http://hdl.handle.net/11449/132637; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/10-12-2015/000854163.pdf

Human vulnerability to HIV infection is not uniform, virological and host factors are determinants on the risk of transmission and natural infection progression. KIR genes polymorphisms have been being associated with progression of HIV infection. Several studies have been performed in mono-infected by HIV-1, but few knwoledge is known about the relation of these polymorphisms in coinfection by HIV/HCV. The purpose of this study was to assess the increasing of the infection by HIV in patients coinfected HIV/HCV, based on clinical, laboratory and virological parameters and correlating KIR genes polymorphisms. The study included 251 samples which were divided into three groups (Group 1: 100 HIV mono-infected; Group 2: 100 mono-infected HCV; Group 3: 51 Co-Infected HIV/HCV). Determination of subtypes (HIV) and genotypes (HCV) was held using RNA sequencing. Polymorphisms definitions of KIR genes were determined by PCR-SSP and HLA were accomplished out by sequencing. Clinical and laboratory data, regarding the evolution of HIV and HCV infection were analyzed from the medical records of patients. The results obtained by this study concerning KIR, 2DL2, 2DL5 and 2DS2 genes demonstrate the state-of-the-art on the correlation of these polymorphisms with evolution of HCV infection in coinfected individuals. The absence of correlation between the polymorphism of KIR genes with progression of HIV-1 infection in co-infected, suggests that in this particular condition, the presence of HIV-1 may influence much more the disease progression by the HCV than the aids development in itself