Estresse oxidativo na anemia falciforme: antioxidantes endógenos como possíveis alvos terapêuticos

• Ano de defesa: 2015
• Autor(a) principal: Silva, Danilo Grünig Humberto da [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Genetica humana; Anemia falciforme; Stress oxidativo; Marcadores genéticos; Melatonina
• Link de acesso: http://hdl.handle.net/11449/127757

Sickle cell anemia (SCA) is characterized by a clinical course highly variable. This feature reflects the complex pathophysiology of SCA which can be affected by a number of modifying factors including polymorphisms associated with clinical aspects and with hydroxyurea (HU) treatment response, and chronic inflammation and oxidative states. Thus, this study aimed to evaluate the influence of genetic markers on oxidative profile os SCA patients. We also correlated these results with hydroxyurea (HU) therapy and with phenotypic manifestations. Moreover we verified a potential direct and indirect antioxidant effects of melatonin (MEL), using a sickle cell suspension as experimental model. After informed consent, the studied groups were divided according to the experimentation manner. Analysis in vivo: the group was composed by 95 SCA patients, regardless gender, and age ranging from 10-59 years old. All subjects were regularly in clinical follow-up in the Blood center of Rio de Janeiro (RJ) and received a prophylactic treatment with folic acid of 5 mg/day since the SCA diagnosis, while 41 (43,2%) of them were under HU treatment...