Análise da expressão das proteínas celulares p53, p16 e ciclina D1 em amostras de tumores penianos

• Ano de defesa: 2014
• Autor(a) principal: Camilo, Henrique Passarelli [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Virologia; Virus do papiloma; Penis - Câncer; Carcinoma de celulas escamosas; Proteinas celulares; Papillomaviruses
• Link de acesso: http://hdl.handle.net/11449/111011

Squamous cell carcinoma of the penis (SCCP) is a relatively rare invasive epithelial tumor with high morbidity due to the disease itself or its treatment. Untreated patients usually die within two years after diagnosis, due to the uncontrolled locoregional recurrence or distant metastases. In the last years, it has been observed that infection by the high risk human papilloma virus (HPV) is one of the main risk factors for SCCP development. It is known that the E6 and E7 viral proteins are associated with tumor development in other HPV associated carcinomas. However, about SCCP, the molecular mechanisms involved in viral replication and its interactions with the host cell are not well understood. The E6 viral protein is linked to the p53 cellular degradation whereas the E7 is linked with destabilization via phosphorylation of Rb protein, in which cyclin D1 and p16 are closely related. The goal of this project was to correlate the high-risk HPV presence with the expression levels of cellular proteins p53, p16 and cyclin D1 in SCCP samples. Comparative study was conducted with 61 samples from patients infected or not with high-risk HPV. It was observed that in high-risk HPV tissues there is down expression of p53 and cyclin D1 and overexpression of p16 when compared to negative samples (p <0.05). There are not many studies on this tumor. This study may contribute to a greater understanding of this tumor, allowing better treatments and prognoses for patients, and contribute to the development of new methodologies for the distinction between lesions associated or not with high-risk HPV