Detalhes bibliográficos
| Ano de defesa: |
2015 |
| Autor(a) principal: |
Domingues, Natália Bertolo [UNESP] |
| Orientador(a): |
Não Informado pela instituição |
| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Estadual Paulista (Unesp)
|
| Programa de Pós-Graduação: |
Não Informado pela instituição
|
| Departamento: |
Não Informado pela instituição
|
| País: |
Não Informado pela instituição
|
| Link de acesso: |
http://hdl.handle.net/11449/145499
|
Resumo: |
Abstract The aim of this study was to evaluate salivary factors related to dental caries and periodontal disease in children with Down syndrome (DS). Forty-one children aged 6- 15 years, being 18 DS and 23 non-syndromic (control) were selected. First, DMFT/dmft and gingival index (IG) were evaluated and stimulated whole saliva was collected. Then, salivary flow (mL/min) was calculated and pH and salivary buffering capacity were measured. Streptococcus mutans and Lactobacillus spp counts were determined after culture on selective medium and Real-time Polymerase Chain Reaction (qPCR) was used for absolute quantification of Porphyromonas gingivalis, Tannerella forsythia and Aggregatibacter actinomycetemcomitans. Total protein dosage (TP) dosage, superoxide dismutase activity (SOD), glutathione peroxidase (GPx) and total antioxidant capacity of saliva (TAOC) were quantified by spectroscopy. Malondialdehyde level (MDA) was quantified by High-performance liquid chromatography (HPLC). Data were analyzed using Mann-Whitney test and Spearman correlation (α = 0.05). There was no difference in DMFT/dmft and GI index between groups. Salivary flow was significantly lower in DS group (p <0.0001). Related to cariogenic and periodontopathogenic bacteria there was no statistically significant diference between groups. A positive correlation between counts of Streptococcus mutans and Lactobacillus spp and the DMFT/dmft index (r=0,621 e r=0,540, respectivelly), was verified only for the DS group. No significant difference between groups was observed for GPx and TAOC, while TP (p<0.0001), SOD (p=0.0002) and MDA (p=0.0016) were higher in saliva of DS group. There was a positive correlation between TP and GI (r=0,4915), and a negative correlation between SOD and salivary flow (r=-0,4751) in DS group. We could conclude that: (1) reduced salivary flow in children with DS may be an important factor in development of caries... (Complete abstract electronico access below) |
