Expressão dos genes MDR-1, TP53, BCL-2 e BAX em tumor venéreo transmissível canino e sua relação com a agressividade e resposta à terapia

• Ano de defesa: 2014
• Autor(a) principal: Flórez, Luis Mauricio Montoya [UNESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Cão - Doenças; Tumores em animais; Expressão gênica; Apoptose; Citotoxicidade; Quimioterapia; Cytotoxicity
• Link de acesso: http://hdl.handle.net/11449/123330; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/06-05-2015/000827166.pdf

Transmissible venereal tumor - TVT has been subject of numerous investigations, despite this, there are gaps that need studies. There TVTs with varying degrees of aggressiveness, so some do not respond to conventional treatment protocols. Implying that there is progressive alteration of their biological profile. Techniques such as cell culture, flow cytometry and molecular biology offer subsidies to identify and quantify these changes, including predicting the biological behavior of the tumor. The objective was to quantify the gene expression BAX, BCL2, TP53 and MDR1 in primary TVT cells and in vitro before and after vincristine, in order to identify changes regarding the aggressiveness and resistance to therapy. 18 samples of TVT canines were obtained to establish 8 primary cultures. After was performed cytotoxicity tests, survival, apoptosis, growth curve and analysis of expression of genes BAX, BCL2, p53 and MDR1. When comparing the TVT cells treated with vincristine, with those who did not receive treatment, there was statistical difference in relation to cytotoxicity rates, survival, and cell cycle phases, G1, Sub G1 and S. About the MDR-1 gene expression, the same difference was observed when comparing cells treated and untreated groups. In this situation, what would be expected at the low expression of the TP53 gene, however, was the opposite, in other words high expression of TP53 gene. In this situation it is believed that there has been a regulatory mechanism of apoptosis. In BAX and BCL-2 genes were not observed statistical difference between the groups, in this case, appears to have not occurred the regulation expected of BAX by TP53. It is believed that there TP53 mutation. Data low reported on TVT. Although initial, data may conclude that are related to tumor malignancy and chemotherapy resistance