Análise imunohistoquímica da proteína interleucina 31 e filagrina e sua relação com o grau de prurido e restauração da barreira cutânea, antes e após uso de solução repositora de lipídios na epiderme de cães com dermatite atópica

• Ano de defesa: 2015
• Autor(a) principal: Barbosa, Laura Carolina [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Cães - Doenças; Dermatite atópica; Pele - Inflamação; Interleucinas; Imunohistoquímica; Skin - Inflammation
• Link de acesso: http://hdl.handle.net/11449/140242; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/27-06-2016/000866649.pdf

Atopic dermatitis is an allergic, inflammatory and pruritic skin disease affecting genetically predisposed dogs. The most common feature is cutaneous rash that significantly impacts the animals' and owners' quality of life. Interleukin-31 (IL-31) is a cytokine involved in the degree of itching in allergies, while filaggrin (FLG) is a protein associated with adequate hydration and keratinization of the stratum corneum. The purposes of this study were to standardize the technique of immunohistochemistry (IHC) for IL-31 and filaggrin and to quantify and associate them to the degree of itching and skin barrier recovery in dogs with atopic dermatitis before and after topical treatment for lipids restitution in the epidermis. All dogs came from the clinical care of the Veterinary Hospital of FMVZ- Unesp Botucatu. There was no significant difference on the expression of IL-31 between samples obtained either from the dorsal or axillary regions of healthy dogs. When atopic dermatitis and healthy animals were compared, expression of IL-31 was noticeable higher in atopic dermatitis animals. IL-31 quantification in the atopic dermatitis animals evidenced significantly lower expression after the use of skin lipids complex in the epidermis. In the case of filaggrin protein, there was no significant difference between its expression when the axillary and cervical sites were compared both in healthy dogs and atopic dermatitis dogs and also when atopic dermatitis dogs were compared before and after treatment. Nevertheless significant difference in fillagrin levels was observed between the cervical and axillary regions of atopic dermatitis dogs when compared to healthy dogs; filagrina levels in healthy animals were larger than in atopic dermatitis dogs. IL-31 results suggest that this cytokine is involved in the physiology of atopic pruritus and that restitution of the skin epidermal barrier is essential so that we can control the pruritus and immunological...