Estudo da carcinogênese bucal experimental utilizando-se o óxido de nitroquinolina (4-NQO) em ratos

Detalhes bibliográficos
Ano de defesa: 2014
Autor(a) principal: Cabrera Ortega, Adriana Alicia [UNESP]
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual Paulista (Unesp)
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://hdl.handle.net/11449/113930
Resumo: The aim of this study was to validate the tissue and molecular changes during the early stages of experimental oral carcinogenesis in rats, using 4-NQO. Were used 20 rats with approximately 4 months of age, randomly divided into control group (n = 10) and treated with 50 ppm of 4- NQO solution dissolved in drinking water (n = 10). The control group animals were sacrificed on the first day of the experiment and the experimental rats were sacrificed after 8 and 12 weeks of treatment. Histological sections from the tongue were stained with H&E or subjected to immunohistochemistry analysis for detection of PCNA, Bcl – 2, SOCS1 and -3, and STAT3. Part of the specimens was used to verify the expression of vimentin, Cdh1, Cdh2 and TWIST1 by RT - qPCR. The results showed that treatment with 4-NQO after 8 weeks caused severe dysplasia and cellular atypia was exacerbation after 12 weeks of treatment. The positivity of antibodies analyzed, with the exception of STAT3 was increased in both experimental periods. The results of this study indicate that treatment with 4-NQO for 8 or 12 weeks enables evaluating experimental epithelial dysplasias both morphological as molecular level.