Análises moleculares de populações de Wasmannia auropunctata Roger, 1863 (Hymenoptera: Formicidae) e da presença de endossimbiontes

• Ano de defesa: 2015
• Autor(a) principal: Silva, Larissa Marin Rodrigues [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Ants; Formiga; Inseto - Controle; Genetica animal; Formiga - Aspectos moleculares
• Link de acesso: http://hdl.handle.net/11449/123719; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/29-05-2015/000832607.pdf

Wasmannia auropunctata is a generalist ant, native of the Neotropical region that has being spread in worldwide scale and when introduced in new areas, many times by human transportation, can cause environmental, economics and health service troubles. Control alternatives and differences between introduced and native populations as well as between clonal and sexual colonies, has increased the interest about this species. The endosymbiont Wolbachia, the most widespread between arthropods, is responsible for many reproductive manipulations in its hosts and has been considered as a possibility of control agent for several species. Thus, the aim of this work was better understand the relationship between these two organisms and increase the molecular profiles of populations in South America. For this purpose, workers of 31 collection points were analyzed by PCR amplification and Sanger s sequencing of the nuclear (28S rDNA) and mitochondrial (COI, IGS, tRNA e COII) genes, as well as wsp and coxA genes from the endosymbiont. The molecular characterization of workers and their endosymbionts and the Network analysis from both showed that there is evidences for believe that: the Leu-tRNA and the IGS between the COI and COII genes could be used to help in the inter and intraspecific distinction of these ants, respectively; the region of 28S rDNA should not be used alone as a marker to the identification of these ants; the samples that presented shorter IGS could represent ancient populations; natural dispersal and human transportation are likely to be occurring between the samples screened; the coxA gene is more appropriate than the wsp gene for detection of Wolbachia in this ants; vertical and horizontal transmission of the endosymbiont can be occurring; two of the alleles found in this work may be involved in ants specialization. Although this work brings new data and interesting hypothesis, more studies are needed to achieve a better...