Reconstruction of molecular networks involved in citokine-induced myotubes atrophy integrating microRNA and mRNA expression

Detalhes bibliográficos
Ano de defesa: 2013
Autor(a) principal: Fernandez Garcia, Geysson Javier [UNESP]
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Dissertação
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual Paulista (Unesp)
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://hdl.handle.net/11449/108576
Resumo: The skeletal muscle atrophy is a common phenomenon in many chronic systemic diseases such a sepsis, chronic heart failure, chronic obstructive pulmonary disease, chronic kidney disease, diabetes, AIDS and cancer. These diseases may be accompanied by a complex metabolic syndrome characterized by muscle wasting, denominated cachexia. The molecular pathways responsible for cachexia are not completely understood, however, evidence suggest that pro-inflammatory cytokines like Tumor Necrosis Factor (TNF)-α and Interferon (INF)-γ have a key role in molecular pathways related to loss of function and muscle mass. The complexity of mechanisms controlling gene expression in this process suggests the involvement of additional regulatory molecules, such as microRNAs; these RNA molecules encoded by the genome regulate the function of skeletal muscle during development and various muscle diseases. MicroRNAs orchestrate common pathways or biological function, this unique feature gives rise as an effective tool for determining the pathways involved in specific diseases or biological processes. The hypothesis of this work is that the muscle atrophy induced by TNF-α and INF-γ has a microRNAs expression profile that allow the identification of regulatory networks and molecular pathways