Desenvolvimento do cultivo 3D a partir de células primarias de neoplasias mamárias caninas: estudo da apoptose sobre efeito ou não da carboplatina

Detalhes bibliográficos
Ano de defesa: 2014
Autor(a) principal: Silva, Daniela Stockmann [UNESP]
Orientador(a): Não Informado pela instituição
Banca de defesa: Não Informado pela instituição
Tipo de documento: Tese
Tipo de acesso: Acesso aberto
Idioma: por
Instituição de defesa: Universidade Estadual Paulista (Unesp)
Programa de Pós-Graduação: Não Informado pela instituição
Departamento: Não Informado pela instituição
País: Não Informado pela instituição
Palavras-chave em Português:
Link de acesso: http://hdl.handle.net/11449/128138
http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/17-09-2015/000850082.pdf
Resumo: In Veterinary Medicine canine mammary tumors have high casuistry and in many cases bad prognosis the prognosis is bad. Thus it is imperative to develop efficient ways to study the behavior of this tumor using tissue culture tests for applying chemotherapeutic associated with expression inducers of apoptosis. The aim of this study was to grow cells of breast tumors in 3D culture where can mimic the environment in vivo. Additionally, analyze the expression of the caspase protein 2, 3, 8, 9, Bcl-2 and Bax and cells after treatment with carboplatin (dose 1.25 mM). Thus, analyse of p53 cells in monolayer culture in high-pass (up to 58 passages) and low-pass (up to 20 passages). The spheroids formed decreased in size after treatment with carboplatin. The expression of Bcl- 2 in both samples showed resistance to carboplatin in two histological types (solid carcinoma and squamous cell carcinoma). The caspase 2 and 3 not expressed in treated samples indicating that carboplatin does not cause cell death by apoptosis. P53 showed cytoplasmic and in the nucleus, indicating that abnormal protein may be associated with malignant progression in cancer. All assays developed in 3D culture showed that it is possible with this technique, further studies on mammary tumors in dogs that can contribute on new discoveries regarding the prognosis and treatments