Características qualitativas de oócitos obtidos por Laparoscopic Ovum Pick-up (LOPU) e da maturação nuclear em ovinos

• Ano de defesa: 2013
• Autor(a) principal: Denadai, Renan [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Ovino; Reprodução animal; Técnicas de maturação in vitro de oócitos; Hormonio foliculo-estimulante; Laparoscopia; Ultrassonografia; In Vitro Oocyte Maturation Techniques
• Link de acesso: http://hdl.handle.net/11449/128043; http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/21-09-2015/000847150.pdf

The present study aimed to evaluate the ovarian response to superstimulation using 80UI FSH and eCG 300UI after ablation LOPU (LOPU1) of all follicles present in both ovaries. Determine mlehor time to make a new LOPU (LOPU2) by ultrasound evaluation of the ovaries every 12 hours LOPU1. And comparing the responses ovarian DLOPU treatment with the treatment Ono Shot adapted Baldassarre (1996) with the DLOPU described here, the amounts of COC and quality of the structures obtained after process and IVM for 24 hours. It was determined the time for completion of LOPU2 in 36 hours after the overstimulation. The mean number of follicles present in the ultrasonographic evaluation immediately before the LOPU in both treatments was about 9 structures did not differ from follicles aspirated media, the recovery rate common to the two treatments was 62.5%. Immediate assessment of the structures obtained as well as after 24 IVM demonstrated that COCs do not hear qualitative difference between treatments