Influência da gonadotrofina coriônica humana sobre a hemodinâmica de folículos pré-ovulatórios e desenvolvimento luteal em águas

• Ano de defesa: 2013
• Autor(a) principal: Romano, Rafael Mide [UNESP]
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Estadual Paulista (Unesp)
• Palavras-chave em Português: Egua - Reprodução; Ovulação; Corpo lúteo; Doppler, Ultrassonografia; Ovulation
• Link de acesso: http://hdl.handle.net/11449/108506

In recent years the hCG, because of its biological LH-like action, has been studied not only to induce ovulation, but also as luteotrophic agent. Doppler ultrasonography is a technique that allows the hemodynamic evaluation of follicular and luteal tissue, in real time, providing important functional information about these structures. This study aimed to describe the hemodynamics changes of pre-ovulatory follicles and characterize the structural and functional development of the corpus luteum of mares treated with different doses of hCG, at different times of estrous cycle. In experiment 1, mares were treated with 750, 1500, 2500 IU of hCG or NaCl 0.9%, intravenously, on presence of pre-ovulatory follicle ≥35mm. Doppler US exams, to evaluate the follicle vascular perfusion, were done every hour during first 12 hours post-treatment. Thereafter, it was done every 6 hours until ovulation. In experiment 2, treatment was performed with 750, 1500 or 2500 IU of hCG to induce ovulation, on the day of ovulation or two days post-ovulation. The luteal area, volume and vascular perfusion, and plasma progesterone concentration were evaluated daily from D0 to D8. No effect of dose and time was detected for follicle vascular perfusion. In experiment 2, there was no effect of dose and moment of treatment for all end points. Throughout the day, all end points presented significant difference, except the luteal area and volume. The hCG-treatment, to induce ovulation, did not increase follicle vascular perfusion post-treatment. hCG administered on the presence of follicle ≥35 mm, on D0 or on D2, had no positive influence on structural and functional development of corpus luteum, independent of dose