Detalhes bibliográficos
| Ano de defesa: |
2015 |
| Autor(a) principal: |
Albuquerque, Yasmin Etienne [UNESP] |
| Orientador(a): |
Não Informado pela instituição |
| Banca de defesa: |
Não Informado pela instituição |
| Tipo de documento: |
Dissertação
|
| Tipo de acesso: |
Acesso aberto |
| Idioma: |
por |
| Instituição de defesa: |
Universidade Estadual Paulista (Unesp)
|
| Programa de Pós-Graduação: |
Não Informado pela instituição
|
| Departamento: |
Não Informado pela instituição
|
| País: |
Não Informado pela instituição
|
| Link de acesso: |
http://hdl.handle.net/11449/145501
|
Resumo: |
The aim of this work was to validatean in vitro caries induction model and use it to evaluate the antimicrobial activity of a mouthrinsecontaining Croton doctorisessential oil.Studies were performed with polymicrobial biofilmdeveloped on the surface of enamel bovine blocks. The blocks were subjected to a semi-continuous regimenof sucrose exposure, in which differentvariables were evaluated. The following parameters were studied:biofilm bacterial concentrationand acidogenicity and percentageof enamel hardnessloss.The essential oil was obtained by hydrodistillation and its minimum inhibitory concentration (MIC) and minimumbactericidal concentration (MBC)were determined by broth microdilution. The developed modelwas applied to test a mouthwashcontaining essential oil at CIM concentration.A mouthwash without essential oil and chlorhexidine digluconate0.12% (CHX) were used, respectively, as negative and positive control. It was established a cariogenic regimenwith 0.5% sucrose and exposure to the treatment solutions for fourdays.The MIC/MBCof the essential oil andCHX were, respectively, 0.625%/1.250% and0.004%/0.008%.There were no significant statistically difference between themouthwash with essential oil action and the negative control in all parameters evaluated (p> 0.05). CHX showed significant differences from control(p <0.001). Using the validated model, it can be concludedthat the incorporation of C.doctorisessential oil to a mouthwash at CIM, is not effective in controlling polymicrobial biofilms growth and enamel desmineralization. 520 |
