Enhanced photosynthetic efficiency in a Solanum pennellii chromosome 2 QTL

• Ano de defesa: 2018
• Autor(a) principal: Costa, Jaciara Lana
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Universidade Federal de Viçosa
• Palavras-chave em Português: Solanum pennellii; Genética; Bioquímica; Variação (Genética); Metabolismo; Fotossíntese; Fisiologia Vegetal
• Link de acesso: https://locus.ufv.br//handle/123456789/27558

Photosynthesis, one of the most important physiological processes, may be limited both by variations in diffusive and biochemical properties. Several studies have demonstrated that the genetic variation in these properties possibility to increase rates of carbon assimilation and plant yield. In a previous study utilizing a Solanum pennellii introgression lines (IL) population of 71 lines, the IL 2-5 and 2-6 displayed photosynthetic rates increased by up to 22% in comparison with control plants. However, understanding the genetic and physiological basis of potential mechanisms involved in the enhanced CO 2 assimilation exhibited by these lines is essential for further biotechnological applications. Thus, this study aimed to uncover the physiological factors involved in the up regulation of photosynthesis in the IL 2-5 and IL 2-6. The ILs have increased photosynthetic capacity followed by higher biomass production when compared to M82. Despite the higher photosynthetic capacity, any difference in relation to stomatal and mesophyll conductance was observed. Nevertheless, the maximum carboxylation velocity, maximum electron transport capacity and chloroplastidic CO 2 concentration correlated positively with carbon isotope composition. Further metabolite analysis also revealed that the higher photosynthetic capacity was associated with higher levels of Rubisco as well as of starch. Analysis of Western blot and data from A/C i curve confirmed that biochemical properties are involved in genetic variation on chromosome 2 which has been related to positive effects on photosynthesis and carbon metabolism.