Wild tomato specie Solanum pennellii as a genetic resource to discover genes of interest to tomato breeding

• Ano de defesa: 2022
• Autor(a) principal: Pessoa, Herika Paula
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Universidade Federal de Viçosa; Fitotecnia
• Palavras-chave em Português: Tomate - Melhoramento genético; Introgressão; Tomate - Resistência à seca; Sementes - Qualidade; Recursos genéticos vegetais; Solanum pennellii; Solanum lycopersicum; Fitotecnia
• Link de acesso: https://locus.ufv.br//handle/123456789/31916; https://doi.org/10.47328/ufvbbt.2022.378

The Solanum pennellii introgression line (IL) population is a valuable source of gene reservoirs for many desirable traits for tomato breeding. Our main goal was to investigate whether S. pennellii introgression fragments carry advantageous genes to increase germination, seedling vigor, and drought tolerance during the vegetative/reproductive stage. If so, we aimed to select promising ILs possessing these traits to be included in tomato breeding programs and investigate their S. pennellii fragments to uncover candidate genes associated with seed germination, seedling vigor, and drought tolerance during the vegetative/reproductive stage. A multi-trait index based on factor analysis and genotype–ideotype distance (FAI-BLUP index) was used to rank the ILs according to seed quality and drought tolerance during the vegetative/reproductive stage. Then, introgressed segments of interest were identified for candidate gene identification using the map of the tomato IL population, as provided by the SOL Genomics Network. The FAI-BLUP index ranked the ILs above their parent cultivar M- 82 for seed germination, seedling vigor, and drought tolerance during the vegetative/reproductive stage, confirming that fragments of the S. pennellii genome can donate genes of interest for these traits. IL 6-1, IL 4-1-1, IL 4-4, IL 11-4-1, IL 1-1-2, and IL 10-1 were closest to the ideotype of seed germination performance and vigor. IL 1-4-18, IL 7-4-1, IL 7-1, IL 7-5-5, and IL 1-2 were ranked closest to the drought-tolerant ideotype during the vegetative/reproductive stage. The genes At1g75350, At2g26350, At2g28490, At3g10920, At5g13200, At5g41480, and At4g21800 are candidate genes for seed germination; At5g53000, At1g46480, At1g30755, At5g47390, At2g29630, and At4g03400 are candidate genes for seedling vigor; and At3g03790, At3g06050, At3g12630, At3g15530, At3g23590, At3g49480, At3g61140, At3g62130, At3g62010, and At3g63520 are candidate genes for drought tolerance in the vegetative/reproductive stage. Because they are already associated with genetic markers, they can be transferred to elite tomato cultivars through marker-assisted technology after validation.Keywords: Solanum lycopersicum. Seed quality. Drought. Water stress. Introgression line. FAI-BLUP.