Genome-wide association analysis for soybean bacterial pustule resistance, functional analysis, and gene networks

• Ano de defesa: 2019
• Autor(a) principal: Fonseca, Pollyanna Capobiango da
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: eng
• Instituição de defesa: Universidade Federal de Viçosa
• Palavras-chave em Português: Xanthomonas axonopodis pv. glycines; Glycine max; Estudo de Associação Genômica Ampla; Melhoramento Vegetal
• Link de acesso: https://locus.ufv.br//handle/123456789/27059

Bacterial pustule of soybean (Xanthomonas axonopodis pv. glycines) is a foliar disease leading to symptoms such as yellow halos. Potential soybean protein-coding genes related to bacterial pustule infection were investigated aiming future application on soybean breeding programs. XAG2440 p and XAG2447 strains were used to inoculate 109 Brazilian cultivars on greenhouse, and the phenotyping data used on association analysis and gene mining. Network analysis was proceeded with two different association analysis, so that genes and transcription factors (TFs) with more connections were highlighted. Cultivars inoculated with XAG2447 and XAG2440 p not presented and presented 82.57% of susceptibility symptoms, respectively. Cultivars genotyped by 6,000 SNPs were used on the association analysis revealing 13 SNPs significantly associated with soybean bacterial pustule. Of these, eight are inside genes – 3 inside coding regions of LOC100779077, histone-lysine N-methyltransferase SUVR4, and ABC transporter B family member 9 proteins, and 5 outside of genes. Despite the fact that association analysis showed different marker regions in linkage disequilibrium, Gene-TFs network provided evidence that key TFs might regulate genes on both association analysis, for example, the highly connected ERF011, WRKY8, WRKY33, and MYB30. In addition, shikimate metabolic process, regulation of peroxisome size, response to ozone, and triglyceride catabolic process appear to play an important role on soybean immunity against the foliar pathogen. These results confirmed that disease screening symptoms of XAG2447 are less aggressive than of XAG2440 p and that soybean pustule resistance is a highly complex trait with defense-related protein-coding candidate genes and TFs possibly acting upon soybean defense mechanisms.