Nanopartículas no controle de mancha bacteriana (Xanthomonas campestris pv. vitians) na cultura da alface

• Ano de defesa: 2024
• Autor(a) principal: Freitas, Natalia Martins
• Tipo de documento: Dissertação
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de Uberlândia; Brasil; Programa de Pós-graduação em Agronomia
• Palavras-chave em Português: Bactéria; Detecção; Emergência; Germinação; Lactuca sativa; Sementes; Bacteria; Detection; Emergence; Germination; Seeds; Nanopartículas; Nanoparticles; CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::CIENCIA DO SOLO::MICROBIOLOGIA E BIOQUIMICA DO SOLO; Agronomia; Pragas agrícolas - Controle; Alface; Horticultura; ODS::ODS 15. Vida terrestre - Proteger, recuperar e promover o uso sustentável dos ecossistemas terrestres, gerir de forma sustentável as florestas, combater a desertificação, deter e reverter a degradação da Terra e deter a perda da biodiversidade.
• Link de acesso: https://repositorio.ufu.br/handle/123456789/44651; http://doi.org/10.14393/ufu.di.2024.5218

Bacterial spot of lettuce caused by Xanthomonas campestris pv. vitians can result in significant losses for the crop. The use of nanoparticles offers a new approach to controlling the disease. This study aimed to investigate the use of nanoparticles in the control of bacterial spot and in the treatment of lettuce seeds. The toxicity of the nanoparticles was assessed by determining the inhibition of bacterial growth in vitro. Lettuce seeds were inoculated with the bacteria and treated with CuO, NiO, ZnO:1Ag and ZnOCl:5Ag nanoparticles, and the detection of the bacteria in the seeds, germination and emergence of the seedlings were evaluated. To control bacterial spot, CuO, NiO, ZnO:1Ag and ZnOCl:5Ag nanoparticles were sprayed on lettuce plants as preventive and curative application. In the preventive application, CuO, NiO, ZnO:1Ag, ZnOCl:5Ag, water and Kocide were sprayed, and 3 days later, the plants were inoculated with a bacterial suspension (108 CFU/mL). In the curative application, the plants were inoculated with the bacteria and, 3 days later, sprayed with the nanoparticles. Disease severity was assessed and the area under the disease progress curve was calculated. Seventeen nanoparticles inhibited bacterial growth in vitro. However, the nanoparticles did not reduce the presence of the bacteria in the seeds, and NiO reduced seed germination and seedling emergence. The preventive application of the nanoparticles CuO, NiO, ZnO:1Ag and ZnOCl:5Ag reduced the severity of bacterial spot on lettuce. The nanoparticles CuO, NiO, ZnO:1Ag and ZnOCl:5Ag are alternatives for the management of bacterial spot on lettuce.