Expressão gênica em células osteobláticas humanas após irradiação de laser de baixa intensidade

• Ano de defesa: 2018
• Autor(a) principal: Bomfim, Fernando Russo Costa do [UNIFESP]
• Tipo de documento: Tese
• Tipo de acesso: Acesso aberto
• Idioma: por
• Instituição de defesa: Universidade Federal de São Paulo (UNIFESP)
• Palavras-chave em Português: Lasers; Osteoblasts; Gene expression; Expressão gênica; Osteoblastos
• Link de acesso: https://sucupira.capes.gov.br/sucupira/public/consultas/coleta/trabalhoConclusao/viewTrabalhoConclusao.jsf?popup=true&id_trabalho=6591262; https://repositorio.unifesp.br/handle/11600/52458

The aim of this study was to evaluate the effects of lowlevel laser, through gene expression, in calcium signaling, bone mineralization and cellular stress mechanisms in human osteoblasts. Cell cultures of osteoblastic lineage hFOB1.19 were cultured and assigned into two groups, control (C; n = 78) and laser (L; n = 78) with n = 6 per day of the experimental period. After maturation, cells of the L group were irradiated with a Gallium and Aluminum Arsenide lowlevel laser device, λ = 808 nm, 200 mW nominal power, power density of 200m W/cm2, energy dose or flow rate of 37J/cm2, area of beam of 0,02mm2, time of 5 s, energy of 1 J, at an application point, daily, for 13 days. Then, cell counts were performed and RNA was extracted, quantified, submitted to cDNA synthesis and quantified by comparative Ct by realtime polymerase chain reaction. The values were submitted to statistical analysis by MannWhitney test with p<0,05. The analysis of the cell count was statistically superior in the L group (37,25x104±22,02) in relation to the control group (22,75x104±7.660) with (p = 0,0259) and the values of 2ΔΔCt presented for the genes S100A6, PMCA, calmodulin and FKBP4 hyper expression on the thirteenth day, whereas osteocalcin presented hypo expression and iNOS remained constant in the L and C groups. It is suggested that the mechanism of action of lowlevel laser is possibly, dosedependent and cumulative and the cell cycle phases are related to human osteoblasts.